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pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223-TXNDC3

Plasmid

#23602

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TXNDC3 (NME8 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRho-Cre

Plasmid

#13779

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Rhodopsin promoter-Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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tTA-IRES-Neo

Plasmid

#16541

Depositor

Bert Vogelstein

Publication

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tTA

Expression

Mammalian

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMB413a

Plasmid

#20058

Depositor

Stephen Sligar

Publication

Springer et al Proc Natl Acad Sci U S A. 1987 Dec . 84(

Insert

Sperm Whale Myoglobin

Expression

Bacterial

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Publication

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A

Plasmid

#8823

Depositor

Judy Lieberman

Publication

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A (GZMA Human)

Tags

(His)6 and Enterokinase site

Expression

Bacterial

Mutation

it mature enzyme.

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV_hSyn1_nLightR

Plasmid

#187180

Purpose

Expresses red norepinephrine indicator nLightR in neuronal cells

Depositor

Tommaso Patriarchi

Publication

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has service

AAV9

Insert

nLightR

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-Flpo

Plasmid

#183535

Purpose

INTRSECT

Depositor

Karl Deisseroth

Publication

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Has service

AAV8

Insert

Flpo

Use

AAV

Mutation

None

Promoter

CaMKIIa

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-EYFP

Plasmid

#137162

Purpose

Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-oScarlet

Plasmid

#137135

Purpose

Viral expression of oScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

oScarlet

Use

AAV

Mutation

E95D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTA-attP

Plasmid

#18939

Depositor

Michele Calos

Publication

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attP

Expression

Bacterial

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJFRC153-20XUAS-IVS-B2::PEST

Plasmid

#32134

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2::PEST

Expression

Insect

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBPhsFlp1

Plasmid

#32148

Purpose

Expresses FLP recombinase in Drosophila

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

hsFlp1

Expression

Insect

Mutation

Aspartic Acid at Amino Acid 5

Promoter

Hsp70Bb promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHMGCS-Luc (aka: pES8)

Plasmid

#14947

Depositor

Axel Nohturfft

Publication

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HMG CoA synthase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Publication

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Aequorea victoria, Human, Fly)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFasBac+GFP-CAMSAP2

Plasmid

#59037

Purpose

for baculovirus expression of CAMSAP2 in Sf9 cells

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJFRC165-20XUAS-IVS-R::PEST

Plasmid

#32142

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

R::PEST

Expression

Insect

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Luc-SIRT1 mutant 3'UTR

Plasmid

#20380

Depositor

Charles Lowenstein

Publication

Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SIRT1 (SIRT1 Human)

Use

Luciferase

Mutation

mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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piRFP-N3-Tyrosinase

Plasmid

#80152

Purpose

Melanosome marker

Depositor

Santiago Di Pietro

Publication

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tyrosinase (TYR Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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