165,688 results

1. mCh-Climp63

   Plasmid

   #136293

   Purpose

   expression of Climp63

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Cytoskeletal-linking protein 63 (Ckap4 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDestattB/CY (pCM327)

   Plasmid

   #68341

   Purpose

   Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

   Depositor

   Christian Mosimann, Leonard Zon

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Blast empty (w263-1)

   Plasmid

   #17486

   Purpose

   3rd gen lentiviral empty control vector, CMV promoter, Blast 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. p28-2iDMD-puro

   Plasmid

   #88895

   Purpose

   28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dystrophin (DMD Human)
   luciferase
   puromycin resistance enzyme
   mCherry
   

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-hSTING-EGFP

   Plasmid

   #214149

   Purpose

   Retroviral transduction of EGFP-tagged STING

   Depositor

   Nan Yan

   Article

   Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM238

   Plasmid

   #161515

   Purpose

   Neuronal expression of histamine-sensitive chloride channel for negative selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

   Insert

   pEXP[Psnt-1 | histamine | rpl-3 UTR]
   

   Expression

   Worm

   Promoter

   Psnt-1

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti.CAG.H2B-Dendra2.W

   Plasmid

   #51005

   Purpose

   Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

   Depositor

   Rusty Lansford

   Insert

   H2B-Dendra2
   

   Use

   Lentiviral

   Tags

   histone2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCMV_HyPer7RgDAAO

   Plasmid

   #217653

   Purpose

   Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Lentiviral

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   CMV

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

   Plasmid

   #183410

   Purpose

   piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   KRAB-dCas9-ecDHFR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXJ-Myc-GATA4

    Plasmid

    #232647

    Purpose

    transient expresses GATA4 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    GATA4 (GATA4 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. hACE2

    Plasmid

    #1786

    Purpose

    Mammalian expression of human ACE2

    Depositor

    Hyeryun Choe

    Article

    Li et al Nature. 2003 Nov 27. 426(6965):450-4.

    Has Service

    Cloning Grade DNA

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. paavCAG-pre-mGRASP-mCerulean

    Plasmid

    #34910

    Purpose

    pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAXEF-Rap1-WT

    Plasmid

    #32697

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Expression

    Mammalian

    Promoter

    EF promoter

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Available Since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CCR5-Tango

    Plasmid

    #66242

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pXL-BacII-CAG-mCherry

    Plasmid

    #170519

    Purpose

    Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

    Insert

    CAG-mCherry
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPdR

    Plasmid

    #85083

    Purpose

    Expresses putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1-GfaABC1D-Rpl22-HA

    Plasmid

    #111811

    Purpose

    Express Rpl22HA in astrocytes

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Ribosomal protein L22 (Rpl22 Mouse)
    

    Tags

    HA

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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