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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN mEGFP

Plasmid

#188643

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Plasmid

#222993

Purpose

Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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E2F-1 wt-pGex2TK

Plasmid

#21668

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

E2F-1 (E2F1 Human)

Tags

GST

Expression

Bacterial

Mutation

wild type

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag Runx1 FL (P#1790)

Plasmid

#14585

Depositor

Matija Peterlin

Article

Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

Insert

Runx1 (Runx1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-Cul7

Plasmid

#20695

Depositor

Yue Xiong

Article

Andrews et al Oncogene. 2006 Aug 3. 25(33):4534-48.

Insert

cullin 7 (CUL7 Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pSUPER-scramble

Plasmid

#118349

Purpose

This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

scrambled shRNA

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-Stat5a1*6

Plasmid

#130668

Purpose

Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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2C-3XtbGFP-PEST

Plasmid

#69072

Purpose

Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

turboGFP

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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IDP425

Plasmid

#87022

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-1xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-KRf-TetR

Plasmid

#216269

Purpose

Construct 2: Lipid binding domain KRφ - TetR

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

Lipid binding motif (KRf) - TetR

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCKmatBC

Plasmid

#138587

Purpose

matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

Depositor

Markus Jeschek

Article

Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

Insert

bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)

Use

Synthetic Biology

Expression

Bacterial

Mutation

matB contains native GUG start codon

Promoter

Plac promoter

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5F Smad2

Plasmid

#12623

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPdelta

Plasmid

#13394

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPdelta (Cebpd Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2B

Plasmid

#23998

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2B (Grin2b Rat)

Tags

NR2B signal sequence (aa1-31) and SEP (supereclip…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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PET30B-PTEN

Plasmid

#20741

Depositor

Alonzo Ross

Article

Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

Insert

PTEN (PTEN Human)

Tags

6xHis

Expression

Bacterial

Available Since

April 2, 2009

Availability

Academic Institutions and Nonprofits only
pBAD_FLAG-GFP(Y39TAG)-6His

Plasmid

#223510

Purpose

Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Insert

GFP(Y39TAG)

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

Y39TAG

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/intron/GFP

Plasmid

#113547

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

GFP

Tags

GFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTIR_Poetur_RFP

Plasmid

#248202

Purpose

Poetur RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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