159,258 results

1. mCherry-2A-eGFP(W58X) pcDNA3.1(-)

   Plasmid

   #172229

   Purpose

   Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   mCherry-2A-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFFLuc-IVT

   Plasmid

   #205147

   Purpose

   IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA

   Depositor

   Igor Ulitsky

   Insert

   FireFly luciferase
   

   Use

   Luciferase; In-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTag-eGFP-SV40

   Plasmid

   #117813

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB517

   Plasmid

   #74859

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.4_17G6-1 heavy chain

   Plasmid

   #198249

   Purpose

   recombinant expression of heavy chain of monoclonal anti-AMP antibody 17G6-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   heavy chain of monoclonal anti-AMP antibody 17G6-1 (mouse IgG2b)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. L4440_BioBrick-hsf-1-sgRNA-A

   Plasmid

   #177786

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hsf-1 (hsf-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-mCherry-ARHGEF2

   Plasmid

   #207959

   Purpose

   Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

   Depositor

   Johanna Ivaska

   Article

   Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_011

   Plasmid

   #59702

   Purpose

   This lentiviral vector can be used to assay Cas9 activity.

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL20_mEGFP_PAX3_FOXO1

    Plasmid

    #205875

    Purpose

    Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PAX3_FOXO1 (PAX3, FOXO1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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