165,688 results

1. pBS-SKII-3XHA-HALO-NAT

   Plasmid

   #188931

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-NAT
   

   Expression

   Yeast

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SPHK2 gRNA (BRDN0001146242)

   Plasmid

   #77095

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-PDGFRb-IRES2-EGFP

   Plasmid

   #204354

   Purpose

   Expresses wild-type PDGFRb gene.

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VPS13B^Halo

   Plasmid

   #224584

   Purpose

   codon optimized Halo tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^Halo (VPS13B Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 809 pcDNA3 GFP PTEN

   Plasmid

   #10759

   Depositor

   William Sellers

   Article

   Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

   Insert

   PTEN (PTEN Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBA560

   Plasmid

   #186236

   Purpose

   pTet-eLbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 1227 pcDNA3 Myr HA Akt2

   Plasmid

   #9016

   Depositor

   William Sellers

   Insert

   Akt2 (AKT2 Human)
   

   Tags

   HA and Myr

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-FRT-TOgw-C-mCherry

   Plasmid

   #223952

   Purpose

   Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1_T6B -mCherry

   Plasmid

   #205600

   Purpose

   expresses human T6B peptide fused with mChery in mammalian cells

   Depositor

   Marc Friedlander

   Article

   Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

   Insert

   T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SYNJ1_BirA_Nterm

    Plasmid

    #221523

    Purpose

    Flag-tagged biotinylation enzyme for co-expression of Synj1

    Depositor

    Alessandro Ori

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Tags

    BirA

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-HA-Ubiquitin-K6

    Plasmid

    #121151

    Purpose

    Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    All Ks apart from K6 mutated to Rs

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

    Plasmid

    #193851

    Purpose

    E1b promoter for transgenic enhancer assays in zebrafish

    Depositor

    Junsu Kang

    Article

    Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Zebrafish expression

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCre-P2A-mCherry

    Plasmid

    #212105

    Purpose

    Expression of Cre and mCherry in mammalian cells

    Depositor

    Daekee Lee

    Insert

    Cre recombinase and mCherry
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pArcCreER

    Plasmid

    #46389

    Depositor

    Liqun Luo

    Article

    Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

    Inserts

    Arc (Arc Mouse)
    CreERT2
    

    Use

    Mouse Targeting

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG1186 Lyn11-ECFP-FIREmate

    Plasmid

    #205168

    Purpose

    Expresses Lyn11-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    Lyn11-ECFP-FIREmate
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPdX

    Plasmid

    #85084

    Purpose

    Expresses putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. actc1b-2PH-PLCd-mKate2

    Plasmid

    #109500

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-2PH-PLCd-mKate2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge