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pZH60 pLIB-spt5

Plasmid

#231648

Purpose

express S.cerevisiae spt5 in insect cells

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

spt5 (SPT5 Budding Yeast)

Expression

Insect

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-ETN-lox2272

Plasmid

#154833

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

ETN

Use

Cre/Lox

Expression

Mammalian

Promoter

100RPU.2

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PAmKate

Plasmid

#32691

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Expression

Bacterial

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

araB

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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YFP-ICP0FxE-C3

Plasmid

#134561

Purpose

Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

YFP

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Dnmt3l

Plasmid

#13356

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-Dest53-SHP-1 WT

Plasmid

#21791

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pEYFP(C1)-PP1alpha

Plasmid

#44231

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1A (PPP1CA Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSG15-pBAD-tetO-deGFP

Plasmid

#102450

Purpose

Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Expression

Bacterial

Promoter

pBAD-tetO

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV2-V5; UAS-mCherry

Plasmid

#173123

Purpose

Expresses uTEV2 and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV2-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUREF-EX

Plasmid

#199331

Purpose

A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

Depositor

Rikiro Fukunaga

Article

Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

Type

Empty backbone

Expression

Mammalian

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-DmrA

Plasmid

#61680

Purpose

GFP-labeled SAH scaffold with DmrA attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-DmrA

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

Plasmid

#66708

Purpose

expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

Depositor

Charles Gersbach

Insert

S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hUbC

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGAL4-CREB

Plasmid

#15221

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB (Creb1 Rat)

Tags

Gal4

Expression

Mammalian

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA 3.3 EGFP

Plasmid

#115786

Purpose

Expresses EGFP in mammalian cells

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y407F

Plasmid

#50504

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y407F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pRK5myc RhoA N19

Plasmid

#15901

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Tags

myc

Expression

Mammalian

Mutation

T19N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Plasmid

#24418

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Use

Mouse Targeting

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-Dppa3

Plasmid

#13365

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 3 (Dppa3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pTol2_LSEC:Cas9green; erbb2_gRNA171

Plasmid

#199338

Purpose

pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

LSEC
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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