165,688 results

1. pDONR223_EGFR_WT

   Plasmid

   #81926

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYeDP60-hATP13A2-WT

   Plasmid

   #171377

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Tags

   BAD tag

   Expression

   Yeast

   Promoter

   URA3

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3_c-MYC

   Plasmid

   #26818

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-c-MYC-3'UTR (MYC Human)
   

   Expression

   Mammalian

   Mutation

   Sequence begins from second ATG; Synthetic UTR se…

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGL003

   Plasmid

   #215335

   Purpose

   amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)

   Depositor

   Gene-Wei Li

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   pSpank

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MGP M155

   Plasmid

   #26527

   Depositor

   David Baltimore

   Article

   O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

   Insert

   miR-155 (Mir155 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pLPC-dnMAML1-mVenus

   Plasmid

   #171023

   Purpose

   Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

   Depositor

   Masashi Narita

   Article

   Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

   Insert

   MAML1 Dominant Negative (MAML1 Human)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   amino acids 12-74 only, hence conferring dominant…

   Promoter

   CMV

   Available Since

   April 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST44

   Plasmid

   #64007

   Purpose

   polycistronic cloning vector for pST44 plasmid suite

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mCherry-LKB1(WT)

   Plasmid

   #84639

   Purpose

   mCherry-tagged, wild-type LKB1

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1 (STK11 Synthetic, Human)
   

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3 2 kb prom. CD274

   Plasmid

   #107003

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. RG6

    Plasmid

    #80167

    Purpose

    fluorescent protein splicing reporter

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    dsRed and GFP
    

    Expression

    Mammalian

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Neo-Bam p53 R248W

    Plasmid

    #16437

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R248W (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R248W

    Available Since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. VGluT1-SNAPtag

    Plasmid

    #107371

    Purpose

    Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    Vesicular Glutamate transporter 1 (Slc17a7 Rat)
    

    Tags

    SNAPtag, 104 aa before C term

    Expression

    Mammalian

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pM-ABE

    Plasmid

    #196292

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding ABE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. RabV CVS-N2c(deltaG)-EGFP

    Plasmid

    #73461

    Purpose

    Expresses EGFP for monosynaptic circuit mapping

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    EGFP
    

    Use

    Neurotropic virus

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP1339

    Plasmid

    #119271

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-Merlin S518D

    Plasmid

    #84295

    Purpose

    Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S518D

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0665

    Plasmid

    #141656

    Purpose

    Lentiviral vector for overexpressing the SCX transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SCX (SCXB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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