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We narrowed to 1,293 results for: Ncl

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Showing: 921 - 940 of 1293 results

  1. pcDNA3.1-TRPV1exCellHalo

    Plasmid
    #220307
    Purpose
    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices
    Depositor
    Eric Senning
    Article
    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231
    Insert
    rTRPV1exCellHalo (Trpv1 Rat)
    Tags
    circularly permutated Halotag (Deo et al., Nat Ch…
    Expression
    Mammalian
    Mutation
    linker included to insert cpHaloTag (
    Promoter
    CMV promoter
    Available Since
    July 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220307-plasmid-map-sequence-id-436649

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  3. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Insert
    Cas9
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Promoter
    none
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  7. pENTR-AtMIR390a-B/c

    Plasmid
    #51778
    Purpose
    Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtMIR390a-B/c
    Use
    Gateway Cloning
    Mutation
    A. thaliana MIR390a precursor sequence including …
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51778-plasmid-map-sequence-id-79411

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  11. pDestTol2CR-QUAS:NTR-mKate2

    Plasmid
    #241914
    Purpose
    Zebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker
    Depositor
    Balázs Enyedi
    Article
    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116
    Insert
    QUAS enhancer driving nitroreductase-mKate2
    Use
    Zebrafish tol2 transgenesis
    Tags
    nitroreductase fused with mKate2
    Promoter
    QUAS
    Available Since
    Oct. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241914-plasmid-map-sequence-id-487159

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  15. cBEST3

    Plasmid
    #234659
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and kasO17tss
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234659-plasmid-map-sequence-id-492747

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  17. pEvolvR-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249069
    Purpose
    Expresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249069-plasmid-map-sequence-id-496321

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  18. pLenti.puro.MCV.ER.RAZ.2

    Plasmid
    #114382
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.
    Depositor
    Reety Arora, Sudhir Krishna
    Article
    Arora et al Microb Pathog. 2019 Dec;137:103778. doi:
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    MCV NCCR (viral own promoter)
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114382-plasmid-map-sequence-id-221614

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  19. pENTR-AtTAS1c-B/c

    Plasmid
    #51774
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor
    Depositor
    James Carrington
    Article
    Carbonell et al Plant Physiol. 2014 Mar 19.
    Insert
    AtTAS1c-B/c
    Use
    Gateway-compatible entry clone
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51774-plasmid-map-sequence-id-79409

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Showing: 921 - 940 of 1293 results