We narrowed to 1,212 results for: Ncl
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Plasmid#102897PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorInsertdCas9VPPH-T2A-GFP-shP53
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.puro.MCV.ER.RAZ.2
Plasmid#114382PurposeThis lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.DepositorInsertMCV NCCR and tumor T antigen regions
UseLentiviralExpressionMammalianPromoterMCV NCCR (viral own promoter)Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
ExpressionBacterialPromoterlacUV5Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
ExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDC123SB-OsMIR390-B/c
Plasmid#61466PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
ExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
JBEI-3716
Plasmid#100169PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertcodon-optimized MK
ExpressionBacterialAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA0055
Plasmid#131774PurposeGuide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer CASS5a guide (gCASS5a) and 5' sgRNA
ExpressionBacterial and YeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VPP300-T2A-GFP-shP53
Plasmid#102896PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.DepositorInsertdCas9VPP300-T2A-GFP-shP53
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:mScarletNLS-SV40pA
Plasmid#155124PurposeTol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:mScarletNLS
UseZebrafish expressionPromoterQUASM1-E1bAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-OsMIR390-B/c
Plasmid#61468PurposeGateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.DepositorInsertOsMIR390-B/c
UseGateway entry vectorMutationO. sativa MIR390 precursor sequence including a c…Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hBnvy
Plasmid#18969DepositorInsertBrevican uncleavable (BCAN Human)
ExpressionMammalianMutationMutation at the ADAMTS cleavage site ESE/SRG: S…Available SinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
DYNLRB1
Plasmid#132533PurposeHuman Rbl-1, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH371-SUP45-N58A
Plasmid#29378DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationN58A mutant gene including genomic sequence 1432 …Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH397-SUP45-I222S
Plasmid#29388DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationI222S mutant gene (corresponding to the sup45-2 a…Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
CDK4-luciferase active (880)
Plasmid#86657PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation867 base pairs including up to coding nucleotide …PromoterCDK5Available SinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH399-SUP45-S30P
Plasmid#29372DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationS30P mutant gene (corresponding to the sl23ts all…Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH375-SUP45-P174Q
Plasmid#29383DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationP174Q mutant gene including genomic sequence 1432…Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T2
Plasmid#97328PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T2
Tags6xHis - TEVExpressionBacterialMutationAddition of two T residues after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBF-E6C
Plasmid#21698DepositorInsertProtein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142) (E6 Human papillomavirus type 16)
TagsProtein G B1 domainExpressionBacterialMutationGB1 (1-56aa) fusion tag with mutations at three p…Available SinceAug. 31, 2009AvailabilityAcademic Institutions and Nonprofits only