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pGEN-mSmoA1

Plasmid

#37674

Depositor

Philip Beachy

Article

Taipale et al Nature. 2000 Aug 31;406(6799):1005-9.

Insert

Smo (Smo Mouse)

Expression

Mammalian

Mutation

W539L

Promoter

CMV

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only

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Filamin-C-stFRET

Plasmid

#61107

Purpose

Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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TfRΔEctoR1-mRFP-GFPnb

Plasmid

#113555

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-mRFP-GFPnb

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMP71-AzamiGreen

Plasmid

#141353

Purpose

pMP71 expression vector containing AzamiGreen

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

AzamiGreen

Use

Retroviral

Expression

Mammalian

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOPTM p62 PB1 (1-102)

Plasmid

#210427

Purpose

Bacterial expression of MBP-PB1(1-102) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-102) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-Tubulin

Plasmid

#31919

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Tubulin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pGEN-mSmo

Plasmid

#37673

Depositor

Philip Beachy

Article

Taipale et al Nature. 2000 Aug 31;406(6799):1005-9.

Insert

Smo (Smo Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pHCMV_MACV_GPC

Plasmid

#207291

Purpose

Mammalian expression of Machupo virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MACV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMyosin-PSmOrange

Plasmid

#31921

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Myosin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pKeratin-PSmOrange

Plasmid

#31924

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Keratin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-CAF1-C-10

Plasmid

#55277

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CAF1

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
lentiGuide-mMaroon

Plasmid

#217008

Purpose

lentiviral plasmid for gRNA cloning with selectable mMaroon expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-N1

Plasmid

#31925

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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Actinin-C-stFRET

Plasmid

#61104

Purpose

Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-SSTR3-N-17

Plasmid

#55326

Purpose

Localization: Cilium, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SSTR3

Tags

mTagBFP2

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pOPTM p62 PB1 ZZ (1-167)

Plasmid

#210429

Purpose

Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 ZZ (1-167) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMP71-mKO2

Plasmid

#141354

Purpose

pMP71 expression vector containing mKO2

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

mKO2

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pYCTK025

Plasmid

#176729

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Lachancea mirantina (receiver part).

Depositor

Victor Sourjik

Article

Krink et al bioRxiv 2021

Insert

ste2Lm

Use

Synthetic Biology

Mutation

Codon-optimized for expression in S. cerevisiae

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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