We narrowed to 1,376 results for: piggyBac

1. LSM043_2 (PB)SFFV-LIDARSensor(mCherry->Casp9-2A-GFP)-hygro

   Plasmid

   #233413

   Purpose

   LIDAR reporter RNA; mCherry as marker, iCasp-P2A-EGFP as output

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   LIDARSensor(mCherry->Casp9-2A-GFP)-hygro
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-EF1A-HALO-hLRRK2

   Plasmid

   #229738

   Purpose

   Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-3xFLAG-SMAD1-active

   Plasmid

   #222579

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SMAD1 (SMAD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   S487E, S489E

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-MGT4-d2EGFP_CMV-mCherry

   Plasmid

   #213764

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

   Insert

   MGT#4-d2EGFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-CLGT3-d2EGFP_CMV-mCherry

   Plasmid

   #213766

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

   Insert

   CLGT#3-d2EGFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPV-C1-crRNA-cloning-EF1a-BA

   Plasmid

   #204623

   Purpose

   piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Antares2-N1

   Plasmid

   #120869

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   Akaluc
   

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-HD252

   Plasmid

   #172656

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD252A/pegRNA-HD252B
   

   Use

   CRISPR

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

   Plasmid

   #162820

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_ORF9c/14
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   CoV2_ORF9c/14(Met1,Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

    Plasmid

    #162822

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b(CoV1h)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b(CoV1h)(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Opie2-PuroR[dsxM]

    Plasmid

    #131616

    Purpose

    Expresses a functional PuroR only in males and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM] (dsx Fly)
    dsRed
    

    Expression

    Insect

    Promoter

    Opie2 and hr5Ie1

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-C3H2B

    Plasmid

    #133387

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-gR-PnGW

    Plasmid

    #133355

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    GFP-NLS
    

    Tags

    HA-tagged GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

    Plasmid

    #124181

    Purpose

    piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Promoter

    UBCmin

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

    Plasmid

    #124180

    Purpose

    piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    EGFP-IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

    Plasmid

    #205565

    Purpose

    Express human NEDD4L (delta C2 isoform)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    delta C2 isoform

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid

    #220143

    Purpose

    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

    Depositor

    Frank Buchholz

    Article

    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

    Insert

    dCas9-Zim3-MeCP2
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    Tre-3G

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-REVERBA-mScarlet-NLS-PEST

    Plasmid

    #190658

    Purpose

    Fluorescent reporter for circadian rhythms

    Depositor

    Mary Teruel

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

    Insert

    Reverba-mScarlet (Nr1d1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LZ10 PBREBAC-H2BHalo

    Plasmid

    #91564

    Purpose

    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

    Depositor

    James Zhe Liu

    Article

    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

    Insert

    histone H2B-HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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