We narrowed to 3,167 results for: tef

Showing: 921 - 940 of 3167 results

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pCfB3044(gRNA XI-2)

Plasmid

#73286

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3046(gRNA XI-5)

Plasmid

#73288

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1-3xHA

Plasmid

#41024

Depositor

Michael Nick Boddy

Publication

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xHA

Use

N-terminal tagging/promoter replacement

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1-GST

Plasmid

#41026

Depositor

Michael Nick Boddy

Publication

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-GST

Use

N-terminal tagging/promoter replacement

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-bleMX6-P41nmt1-3FLAG

Plasmid

#19352

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

bleMX6

Expression

Yeast

Available since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pGS_107

Plasmid

#160650

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Publication

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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cmv-mCherry-8xPP7

Plasmid

#185795

Purpose

mCherry labeled with 8xPP7

Depositor

Robin Lee

Publication

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cmv-mCherry-8xPP7

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHES873

Plasmid

#87945

Purpose

Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)

Expression

Yeast

Promoter

GAL1

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pK3FS

Plasmid

#85776

Purpose

N-ICE plasmid pK3FS

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3047(gRNA XII-1)

Plasmid

#73289

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Expression

Mammalian and Yeast

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-SKII-URA-GAL1-HALO-3xHA

Plasmid

#188937

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-HALO-3xHA

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAG32-3HA-3FLAG

Plasmid

#154081

Purpose

For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

Depositor

Cornelia Kilchert

Publication

Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

Insert

3xHA-3xFLAG-hygR

Use

Pcr-based tagging in yeast

Tags

3xHA-3xFLAG

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Publication

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Expression

Yeast

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3053(gRNA X-2, XI-5, XII-4)

Plasmid

#73295

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Publication

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pVW300

Plasmid

#139495

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pTEF PP7∆FG-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pTEF

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Expression

Bacterial and Yeast

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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