We narrowed to 65,944 results for: des.1

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pAM5411

Plasmid

#132663

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Expression

Bacterial

Promoter

PaadA and PconII

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-hESRRB

Plasmid

#60434

Purpose

Over-expression of human ESRRB

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

ESRRB (ESRRB Human)

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Lin28

Plasmid

#60345

Purpose

Mammalian Expression, Lentiviral

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Lin28 (Lin28a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-PARP1_D770A-mCherry

Plasmid

#192260

Purpose

Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

Depositor

Michael Cohen

Article

Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

Insert

PARP1_D770A (PARP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D770A

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1_R878A-mCherry

Plasmid

#192261

Purpose

Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

Depositor

Michael Cohen

Article

Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

Insert

PARP1_R878A (PARP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R878A

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kir6.1 K+ channel [N366/60R]

Plasmid

#188206

Purpose

Mammalian Expression Plasmid of anti-Kir6.1 K+ channel (Rat). Derived from hybridoma N366/60

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kir6.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnj8 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K39/25]

Plasmid

#182067

Purpose

Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986H

Plasmid

#174801

Purpose

Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986H (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986S

Plasmid

#174802

Purpose

Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986S (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn3

Plasmid

#174806

Purpose

Bacterial expression of isolated PARP1 Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G972R

Plasmid

#174800

Purpose

Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G972R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

G972R, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRRG43-sFRP1

Plasmid

#99071

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea sFRP1 (secreted frizzled related protein 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

sFRP1 (secreted frizzled related protein 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

Plasmid

#46602

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-24 Decoy (Mir24-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

Plasmid

#60418

Purpose

Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

mTiam1-tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

mTiam 64-437 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-tTA2-WPRE-pA

Plasmid

#192070

Purpose

Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B KD/MD

Plasmid

#104450

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

changed K87 to D and M89 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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