We narrowed to 11,803 results for: nsf

1. P41-bio

   Plasmid

   #47739

   Purpose

   Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised P41
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPPAT-11AA-2XStrep

   Plasmid

   #125680

   Purpose

   expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

   Insert

   PPAT (PPAT Human)
   

   Tags

   2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   the first eleven residues have been removed

   Promoter

   CMV

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-sbGLuc-CheRiff-EGFP

   Plasmid

   #114108

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

   Insert

   Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS1993

   Plasmid

   #49123

   Purpose

   AAV plasmid with OLIG1 (Ple304) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple304-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   OLIG1

   Available Since

   May 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEMS2158

   Plasmid

   #70119

   Purpose

   Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-MCS-EmGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CNTF

   Plasmid

   #195338

   Purpose

   Expression of CNTF in mammalian cells

   Depositor

   Kevin Park

   Article

   Yungher et al Gene Ther. 2015 Oct;22(10):811-21. doi:

   Insert

   Ciliary neurotrophic factor (CNTF Human)
   

   Use

   AAV

   Tags

   NGF signal peptide

   Expression

   Mammalian

   Promoter

   UbC promoter with beta-globin intron

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-crRNA(Non-targeting)

   Plasmid

   #109324

   Purpose

   AAV vector expressing non-targeting AsCpf1 crRNA

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-IFITM3-F8-TAG-C72A

   Plasmid

   #122477

   Purpose

   expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 8 to amber codon, cysteine …

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-Pcdh15_mini_V7-CD2

   Plasmid

   #199186

   Purpose

   An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

   Depositor

   David Corey

   Article

   Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

   Insert

   mmPcdh15_mini_V7-CD2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   EC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-Pcdh15_mini_V8-CD2

    Plasmid

    #199187

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    mmPcdh15_mini_V8-CD2
    

    Use

    AAV

    Mutation

    EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV_Sox2 HMEJ donor

    Plasmid

    #97322

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Sox2 HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. FB007

    Plasmid

    #119705

    Purpose

    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Promoter

    gpdA

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-DYRK4-2A-GFP

    Plasmid

    #118288

    Purpose

    Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and EGFP

    Expression

    Mammalian

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SIN-CMV-Cas9-V5-WPRE

    Plasmid

    #87904

    Purpose

    To produce lentiviral vector for gene editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    Cas9-V5
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

    Plasmid

    #135564

    Purpose

    An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    miR-30 FF3
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    SYN1 and hSYN1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMADM-alpha

    Plasmid

    #36890

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    ATG (T)
    GFP
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, deleted…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-SaCas9-U6-sgGabrg1

    Plasmid

    #124856

    Purpose

    Mutagenesis of Gabrg1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg1 (Gabrg1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120393

    Purpose

    pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Insert

    Diras2 shRNA (Diras2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    mCherry.3XFLAG

    Promoter

    U6

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC8_MF-PH-Flag-Nter (VE5627)

    Plasmid

    #139773

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal Flag tag
    

    Tags

    Flag

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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