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pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122

Plasmid

#186982

Purpose

mammalian expression of cas7-11-r1045-gggs-r1122

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122

Expression

Mammalian

Mutation

r1045-gggs-r1122 deletion

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII

Plasmid

#80864

Purpose

Phagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5C6G7G,P

Expression

Bacterial

Mutation

238G to 238D (please see depositor comment below)

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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CAV1-D67G-HA

Plasmid

#27706

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)

Tags

HA

Expression

Mammalian

Mutation

mutant D67G

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#87560

Purpose

Expresses aa397-423 of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa397-423

Use

Cre/Lox and Synthetic Biology

Tags

PYL

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#87559

Purpose

Expresses aa1-396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa1-396

Use

Cre/Lox and Synthetic Biology

Tags

ABI

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HA-Amida

Plasmid

#19794

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

Amida (TFPT Human)

Tags

2XHA

Expression

Mammalian

Available Since

Nov. 24, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA-NLS-ZapCmR1.1

Plasmid

#59011

Purpose

Nuclear zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NLS-ZapCmR1.1 genetically encoded Zn(II) sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pDGE662

Plasmid

#153260

Purpose

M6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE664

Plasmid

#153262

Purpose

M7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE659

Plasmid

#153257

Purpose

M4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE661

Plasmid

#153259

Purpose

M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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ZP62 Cystathionine beta-synthase

Plasmid

#16880

Depositor

Randall Moon

Article

Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Insert

cystathionine beta-synthase (cbsb Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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BacPAK Flag-NFRKB

Plasmid

#19791

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

Flag and His

Expression

Insect

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Pediculus humanus corporis PINK1 (143 - 575)

Plasmid

#110751

Purpose

Bacterial expression of PhPINK1 (crystallography)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Tags

N-His6-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEE389

Plasmid

#149287

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pEE387

Plasmid

#149285

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_FT:tNos

Expression

Plant

Promoter

35S

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 WT

Plasmid

#66139

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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NET39 mRFP-C1 (1219)

Plasmid

#62042

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET39 (PPAPDC3 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Tags

GST and thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK TTLL11 3'UTR

Plasmid

#19857

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

TTLL11 3'UTR (TTLL11 Human)

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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