We narrowed to 13,211 results for: CAR

1. pGTag-NLS-eGFP-B-actin

   Plasmid

   #117812

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

   Plasmid

   #160621

   Purpose

   Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   U6-26:gRNA 4 (pNos): 2.1 aptamer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9 (GB1079)

   Plasmid

   #75399

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-TetOminiCMV-SM

   Plasmid

   #136578

   Purpose

   Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Sox2-E2A-cMyc (Sox2, Myc Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

   Plasmid

   #102594

   Purpose

   Lentivector to express STING V155M delta 342 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M delta 342

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgALK(A)

   Plasmid

   #85562

   Purpose

   Expresses sgRNA targeting human ALK around codon F1174

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   anaplastic lymphoma receptor tyrosine kinase (ALK Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. Snap25-LSL-2A-GFP targeting vector

   Plasmid

   #61575

   Purpose

   Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Snap25-LSL-2A-GFP
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJHG003

   Plasmid

   #126454

   Purpose

   Expresses Cas9-XTEN-dTdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-XTEN-dTdT (DNTT Human, Streptococcus pyogenes)
   

   Expression

   Mammalian

   Mutation

   Added D343E and D345E to inactivate TdT.

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

   Plasmid

   #99746

   Purpose

   Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

   Insert

   Stx3 (STX3 Human)
   

   Tags

   myc-myc-his

   Expression

   Mammalian

   Mutation

   Made six (6) silent mutations to confer resistanc…

   Promoter

   CMV/TO

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65453

    Purpose

    Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV1

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    tetO

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO_FLAG.Ollas.VSVg_NGFR

    Plasmid

    #158324

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG.Ollas.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

    Plasmid

    #47905

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

    Plasmid

    #171952

    Purpose

    All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3

    Depositor

    Sujatha Jagannathan

    Article

    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

    Insert

    serine and arginine rich splicing factor 3 (SRSF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized, truncated at amino acid 114 with…

    Promoter

    TRE

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. VN-PTEN

    Plasmid

    #89331

    Purpose

    expresses hPTEN fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Marciniak et al J Exp Med. 2017 Aug 7;214(8):2257-2269.

    Insert

    PTEN1 (PTEN Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDECKO_GFP_AAVS1

    Plasmid

    #141211

    Purpose

    pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_S.FLAG.VSVg_NGFR

    Plasmid

    #158345

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.FLAG.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO_mCherry_AAVS1_1801

    Plasmid

    #159090

    Purpose

    targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_HA.NWS.V5_NGFR

    Plasmid

    #158248

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.NWS.V5

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIP GFP-MOSPD2 W201E

    Plasmid

    #186471

    Purpose

    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    W201E (no more binding to lipid droplets)

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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