We narrowed to 62,227 results for: cloning
-
Plasmid#71564PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeastDepositorInsertdelta12 fatty acid desaturase (form 3)
ExpressionBacterial and YeastPromoterGAL1 promoterAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK272
Plasmid#71562PurposeProduces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
ExpressionBacterial and YeastPromoterGAL1Available SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MXS_loxP
Plasmid#62430PurposeMXS_chaining vector with loxPDepositorInsertloxP site
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_linker
Plasmid#62431PurposeMXS_chaining vector with linkerDepositorInsertLinker 12AA Gly-/Ser-rich
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGI000
Plasmid#48863PurposeEmpty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.DepositorTypeEmpty backboneUseGolden gate compatible cloning vectorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO SSA1
Plasmid#19462DepositorInsertSSA1 (SSA1 Budding Yeast)
ExpressionMammalianAvailable SinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJW1583
Plasmid#121054PurposeGFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertGFP^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized GFP, and auxin…ExpressionWormAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB508
Plasmid#74850PurposeGateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
B52 + SPRTN sgSTOP
Plasmid#100709PurposeB52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting SPRTN (cloned using BbsI)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCS-TurboID MSCV-IRES-GFP
Plasmid#207957PurposeRetroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminusDepositorInsertTurboID
UseRetroviral; Turboid biotin proximity ligaseTagsTurboIDExpressionMammalianAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-HA
Plasmid#119751PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.DepositorTypeEmpty backboneTagsGST and HAExpressionBacterialPromotertac promoterAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MZB174
Plasmid#228487Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)-GST-HRVExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
R777-E352 Hs.TP53-nostop
Plasmid#70636PurposeGateway ORF clone of human TP53 [NM_000546.5] without stop codon (for C-terminal fusions)DepositorInsertTP53 (TP53 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E355 Hs.TSC2
Plasmid#70639PurposeGateway ORF clone of human TSC2 [NM_001114382.1] with stop codon (for native or N-terminal fusions)DepositorInsertTSC2 (TSC2 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E323 Hs.SPRED2
Plasmid#70607PurposeGateway ORF clone of human SPRED2 [NM_181784.2] with stop codon (for native or N-terminal fusions)DepositorInsertSPRED2 (SPRED2 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E348 Hs.TIAM1-nostop
Plasmid#70632PurposeGateway ORF clone of human TIAM1 [NM_003253.2] without stop codon (for C-terminal fusions)DepositorInsertTIAM1 (TIAM1 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E315 Hs.SHOC2
Plasmid#70599PurposeGateway ORF clone of human SHOC2 [NM_007373.3] with stop codon (for native or N-terminal fusions)DepositorInsertSHOC2 (SHOC2 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E233 Hs.RASAL1
Plasmid#70517PurposeGateway ORF clone of human RASAL1 [NM_001193521.1] with stop codon (for native or N-terminal fusions)DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E249 Hs.RASGRP4
Plasmid#70533PurposeGateway ORF clone of human RASGRP4 [NM_001146202.1] with stop codon (for native or N-terminal fusions)DepositorInsertRASGRP4 (RASGRP4 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E167 Hs.PIK3CD
Plasmid#70451PurposeGateway ORF clone of human PIK3CD [NM_005026.3] with stop codon (for native or N-terminal fusions)DepositorInsertPIK3CD (PIK3CD Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only