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We narrowed to 63,768 results for: cloning

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Showing: 9441 - 9460 of 63768 results

  7. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Publication
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  8. pY036_ATP1A1_G5_Array

    Plasmid
    #86620
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86620-plasmid-map-sequence-id-168857

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  11. pDarmo.CMVT_v1

    Plasmid
    #133072
    Purpose
    Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.
    Depositor
    Kacper Rogala, David Sabatini
    Publication
    Rogala et al Science. 2019 Oct 25;366(6464):468-475.
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    3C-Flag-TwinStrep and TwinStrep-Flag-3C
    Expression
    Mammalian
    Promoter
    CMVT (Enhanced CMV)
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133072-plasmid-map-sequence-id-298087

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  12. pRS143

    Plasmid
    #122377
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Publication
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122377-plasmid-map-sequence-id-241241

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  16. MoClo adapted pUC57-Amp Level 1 position 1

    Plasmid
    #109220
    Purpose
    Non binary level 1 position 1 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants
    Depositor
    Elena Caro Bernat
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109220-plasmid-map-sequence-id-223586

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  17. Dendra1-C2

    Plasmid
    #54724
    Purpose
    Localization: C2 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Dendra1
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mGeos2-VEL-C1

    Plasmid
    #54541
    Purpose
    Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mGeos2-VEL
    Expression
    Mammalian
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. wtKikGR-N1

    Plasmid
    #54718
    Purpose
    Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    wtKikGR
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. Phamret-N1

    Plasmid
    #54641
    Purpose
    Localization: N1 Cloning Vector, Excitation: 458 / 475, Emission: 458 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Phamret
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9441 - 9460 of 63768 results