We narrowed to 17,543 results for: Emb
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Plasmid#127403PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k
Plasmid#127407PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon, K305H,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k
Plasmid#127401PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
TagssfGFPExpressionBacterialPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC/mCitrine/erg3-mCherry
Plasmid#117993PurposeCo-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoterDepositorInserterg3-mCherry
UsePlant/bacteria binary vectorExpressionPlantPromoterGal10Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
TagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-d80
Plasmid#114025PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d80
Plasmid#114030PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S15D
Plasmid#114031PurposeInducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell linesDepositorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu
Plasmid#112999PurposeFor protein expression and purification of full-length Drosophila gnuDepositorInsertfull length gnu (gnu Fly)
ExpressionBacterialAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ATG-3xHA-S11
Plasmid#108238PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plantsDepositorInsertATG-3xHA-S11
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1C389S-HLx8
Plasmid#108140PurposeInactive SAC1 with extended linkerDepositorInsertmCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sph1-VC
Plasmid#89756Purposeexpresses human Synphilin fused to the C-terminal part of VenusDepositorAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-peripherin-CTS
Plasmid#86070PurposeA bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.DepositorInsertPeripherin (prph2.S Frog)
TagsGSTExpressionBacterialMutationcontains the region from 317-336 of peripherinPromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-prRDH-CTS
Plasmid#86069PurposeA bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.DepositorInsertRetinol dehydrogenase (RDH8 Bovine)
TagsGSTExpressionBacterialMutationthe insert is 297-312 of RDH8 (Bos taurus)PromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)
Plasmid#86922PurposeTo express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
TagsFLAG and GFPExpressionMammalianMutationResidues from 90-157 is deletedPromoterCMVAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD1cR
Plasmid#86970PurposeExpresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, G108C in PCNA1PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD2cX
Plasmid#86968PurposeExpresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coliDepositorInsertPCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…PromoterT7 promoterAvailable SinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha1_SF (GB0106)
Plasmid#68232PurposeTwister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAct2 (GB0210)
Plasmid#68191PurposeProvides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid partDepositorInsertAtAct2 terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only