We narrowed to 9,906 results for: UTY
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Plasmid#122229PurposeRescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.DepositorInsertPCDHGC5 (Pcdhgc5 Rat)
UseTags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianMutationSilent mutations are in five consecutive codons …PromoterAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M-L273D-L276D
Plasmid#114183PurposeORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationV107M/L273D/L276DPromoterAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRER-P2A-GFP-PGK-Puro
Plasmid#110862PurposeLentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)DepositorInsertCas9-VRER
UseLentiviralTags3X FLAGExpressionMutationD1135V, G1218R, R1335E, T1337RPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-P2A-GFP-PGK-Puro
Plasmid#110863PurposeLentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGExpressionMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8.A18T
Plasmid#99360PurposeBacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsExpressionBacterialMutationAlanine 18 to ThreoninePromoterT7Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8.E31K
Plasmid#99361PurposeBacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.DepositorInsertcdc8 (cdc8 Fission Yeast)
UseTagsExpressionBacterialMutationGlutamic acid 31 to LysinePromoterT7Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro dn-cHSF1
Plasmid#134732PurposeLentiviral expression vector for constitutively active dominant-negative HSF1DepositorInsertdn-cHSF1 (HSF1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of amino acids 186-202, 379−529PromoterCMVAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Zeo dn-cHSF1
Plasmid#134734PurposeLentiviral expression vector for inducible expression of a constitutively active dominant-negative HSF1DepositorInsertdn-cHSF1 (HSF1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of amino acids 186-201, 380−529PromoterCMV/TOAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pDest40 DHFR.dn-cHSF1
Plasmid#134735PurposeMammalian expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.dn-cHSF1 (HSF1 Human)
UseTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202, 379−529PromoterCMVAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116
Plasmid#123243PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterPGKAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Plasmid#68345PurposeA Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expressionDepositorInsertspuromycin
hCas9
UseCRISPR; Flp/frtTagslinked to red flouresent protein gene mKateII and…ExpressionMammalianMutationPromoterCAGAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA3 (pLP305)
Plasmid#196992PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116A
Plasmid#123245PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationGlycine 116 to AlaninePromoterPGKAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)
Plasmid#62580PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseTagsExpressionMutationmiR-21 binding site 1 mutant (TAAGCTA --> TAGA…PromoterAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)
Plasmid#62581PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseTagsExpressionMutationmiR-21 binding site 2 mutant (TAAGCTA --> TAGA…PromoterAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin
Plasmid#99278PurposeDual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mVenus-IRES-mCherry
Puro
UseLentiviralTagsH2B, IRES, PGK, mCherry, and mVenusExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET01-TAF5-ex8-Minigene-SRSF1 motif MUT
Plasmid#218973PurposeVector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)DepositorInsertTAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
UseTagsExpressionMammalianMutationmutant SRSF1 binding motif (AGTCTCCT)PromoterRSV LTRAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
3` CC: Hygro – H2B-GFP-N – loxP – mScarlet
Plasmid#219565Purpose3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosomeDepositorInsertHygroR_2A_H2B-GFP-N_loxP_mScarlet
UseUnspecifiedTagsExpressionMutationPromoterEF-1aAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-6HIS-TEV-mEGFP,IRES-NGFR
Plasmid#164521PurposeAll-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc IRES neo
Plasmid#98294PurposeFor constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance geneDepositorInsertsFirefly luciferase
IRES
Neomycin resistance gene (NeoR)
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only