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pET22b-CPDSacI

Plasmid

#38255

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet8

Plasmid

#107449

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met8

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-c

Plasmid

#17592

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-c (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–93, 175–942

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pICH41308::hCas9

Plasmid

#49770

Purpose

Level 0 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

hCas9

Use

CRISPR

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-5

Plasmid

#221479

Purpose

Gateway cloning of NKX2-5 overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Gateway donor vector

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB103-Citrine

Plasmid

#79137

Purpose

Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

Depositor

Yutaka Kodama

Article

Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

Insert

Citrine

Tags

Citrine

Expression

Plant

Promoter

MpEF1a promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti puro HA-LMNB1

Plasmid

#227591

Purpose

Lentiviral plasmid expressing human LMNB1 protein

Depositor

Melina Fan, Addgene Research Program

Insert

LMNB1 (LMNB1 Human)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_HD

Plasmid

#90416

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_HD

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pJYDC2

Plasmid

#162459

Purpose

Yeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

ALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR PKAKTRn-Clover

Plasmid

#177199

Purpose

Gateway entry vector encoding PKAKTRnew-Clover

Depositor

Markus Covert

Article

Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

Insert

PKAKTRnew-mClover

Expression

Bacterial

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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ttHsCas12a

Plasmid

#182385

Purpose

Gives high editing efficiency in barley.

Depositor

Wendy Harwood

Article

Lawrenson et al bioRxiv 2022.04.28.489853

Insert

ttHsCas12a

Use

CRISPR; Coding sequence only

Mutation

D156R

Promoter

None

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEpic_Lite

Plasmid

#84373

Purpose

3rd-generation lentiviral destination vector for gateway cloning

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAW12

Plasmid

#110224

Purpose

integration of ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Hyg

Plasmid

#100559

Purpose

hgRNA-A21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet1

Plasmid

#107444

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met1

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57 iLOV CHLAMY

Plasmid

#26769

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Cloning vector

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only
NTERY00

Plasmid

#184109

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY00

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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NTERY02

Plasmid

#184120

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY02

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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P-BT1763

Plasmid

#188111

Purpose

pBolux harboring the promoter region upstream of BT1763

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT1763 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT1763

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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