We narrowed to 3,399 results for: FRI;

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Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBS3Clux

Plasmid

#55172

Purpose

lux-reporter vector, integration at sacA, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-EF1afull-H2B-mCherry-P2A-Fluc

Plasmid

#207253

Purpose

Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

H2B-mCherry-P2A-Fluc

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha_full

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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acGFP WC visual barcode

Plasmid

#158672

Purpose

tagging clonal populations with whole cell acGFP fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

acGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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acGFP NES visual barcode

Plasmid

#158673

Purpose

tagging clonal populations with NES acGFP fluorescent protein. Adding NES to acGFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

acGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.CMV.flex.hM3Dq.Cherry

Plasmid

#170360

Purpose

Cre-inducible lentiviral vector expressing hM3Dq

Depositor

Johan Jakobsson

Article

Klawonn et al Immunity. 2021 Feb 9;54(2):225-234.e6. d

Insert

flex.hM3Dq.Cherry

Use

Lentiviral

Promoter

CMV

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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XE118 CAMKII-T286D-CS2+

Plasmid

#16736

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-T286D (Camk2a Rat)

Use

Xenopus expression

Mutation

threonine 286 mutated to aspartate

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
PxylA

Plasmid

#55178

Purpose

Xylose-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

xylA promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

xylA

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLJC2-MTHFD1-3xFLAG

Plasmid

#217429

Purpose

Lentiviral overexpression of human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

MTHFD1 (MTHFD1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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gCOTS-pyl

Plasmid

#92048

Purpose

This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

Depositor

Lital Alfonta

Article

Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

Inserts

Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)

Use

Cyanobacteria s. elongatus genome recombination i…

Promoter

LeuP (native S. elongatus promoter) and PrcbL

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6f

Plasmid

#112000

Purpose

For enriched expression of GCaMP6f in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6f

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV Fcgamma RIIa IRES neo

Plasmid

#14948

Depositor

Axel Nohturfft

Article

Trivedi et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Fcgamma RIIa (FCGR2A Human)

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 WC visual barcode

Plasmid

#158666

Purpose

tagging clonal populations with whole cell mTAGBFP2 fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAP118

Plasmid

#105495

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

HupA-SmBiT/HupA-LgBiT

Use

Atc-dependent expression in c. difficile

Promoter

Ptet

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
EYFP WC visual barcode

Plasmid

#158675

Purpose

tagging clonal populations with whole cell EYFP fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

EYFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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iNicSnFR12

Plasmid

#227142

Purpose

Genetically encoded fluorescent biosensor for nicotine detection

Depositor

Henry Lester

Article

Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Insert

iNicSnFR12

Tags

6xHis-HA and Myc

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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mTurquoise2 NES visual barcode

Plasmid

#158670

Purpose

tagging clonal populations with NES mTurquoise2 fluorescent protein.

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTurquoise2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s

Plasmid

#111262

Purpose

For enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6s

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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plko-shmfn2-mito-GFP-ER tethering

Plasmid

#160509

Purpose

Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mito-GFP-ER tethering

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2 WC visual barcode

Plasmid

#158669

Purpose

tagging clonal populations with whole cell mTurquoise2 fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTurquoise2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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EYFP NES visual barcode

Plasmid

#158676

Purpose

tagging clonal populations with NES EYFP fluorescent protein. Adding NES to EYFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

EYFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mTurquoise2 Peroxisome visual barcode

Plasmid

#158671

Purpose

tagging clonal populations with Peroxisomal mTurquoise2 fluorescent protein.

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTurquoise2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 NES visual barcode

Plasmid

#158667

Purpose

tagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Ago1

Plasmid

#21534

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pAD/CMV/V5-DEST-Cdc42-2G

Plasmid

#68812

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Adenoviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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PlepA

Plasmid

#55175

Purpose

Strong constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

lepA promoter

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGI3EM31

Plasmid

#135491

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCerulean3

Tags

mCerulean3

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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