2,226 results

1. Myc-gamma3 (in pCAGGS)

   Plasmid

   #118042

   Purpose

   GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG3 (Gabrg3 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FH95pUGW (B3)

   Plasmid

   #74012

   Purpose

   lentiviral expression of EGFP and Psd95 shRNA

   Depositor

   Robert Malenka, Weifeng Xu, Oliver Schluter

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   Psd95
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. XE182 rat Frizzled-1-HA-pCD

   Plasmid

   #16818

   Depositor

   Randall Moon

   Insert

   frizzled-1 (Fzd1 Rat)
   

   Use

   Xenopus expression

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-GFAP-jGCaMP7c variant 1513

   Plasmid

   #173159

   Purpose

   Expresses jGCaMP7c variant 1513 in astrocytes

   Depositor

   Vincent Vialou, Hervé Chneiweiss

   Insert

   jGCaMP7c variant 1513-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. rat APOBEC1-EGFP

   Plasmid

   #112857

   Purpose

   plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

   Depositor

   Silvestro Conticello

   Article

   Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

   Insert

   apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEAK10-His-Myc-Kal9

   Plasmid

   #25441

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal9
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pR-BAM

   Plasmid

   #22649

   Depositor

   Lynn Hudson

   Article

   Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

   Insert

   Myelin Transcription Factor 1L (Myt1l Rat)
   

   Use

   Cloning and rna synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pN1 VAMP2 R125TAG

   Plasmid

   #69877

   Purpose

   express in mammalian cells for unnatural amino acid incorporation into VAMP2

   Depositor

   Silvio Rizzoli

   Article

   Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Amber and Ochre stop codons in the former linker …

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

9. mTFP1-Lysosomes-20

   Plasmid

   #55495

   Purpose

   Localization: Lysosome Membrane, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   LAMP1
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pORANGE Cacnb3-GFP KI

    Plasmid

    #139662

    Purpose

    Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE Cacnb4-GFP KI

    Plasmid

    #139663

    Purpose

    Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-miniSOG-RatLC3

    Plasmid

    #55095

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LC3
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. Geph UTR shRNA

    Plasmid

    #121070

    Purpose

    shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. peGFP-N2-rPrmp

    Plasmid

    #22735

    Depositor

    Lynn Hudson

    Article

    Nielsen et al J Neurosci. 2006 Sep 27. 26(39):9881-91.

    Insert

    Proline-rich transmembrane protein
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Jag1ICD-HA pBacPAK8-3

    Plasmid

    #17331

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Jagged1 (Jag1 Rat)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pR2Q306c

    Plasmid

    #101027

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Use

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSG5-FLAG-CaMKKbeta rat RP deletion

    Plasmid

    #32452

    Depositor

    Anthony Means

    Article

    Anderson et al Cell Metab. 2008 May;7(5):377-88.

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deleted RP domain (amino acids 203-225 missing); …

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Rat SERCA2b (pMT2)

    Plasmid

    #75184

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, Gary Shull

    Article

    Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

    Insert

    ATP2A2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. HT-Pit1

    Plasmid

    #24060

    Depositor

    Frederick Stanley

    Insert

    PIT1 (Pou1f1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

20. CALNL-DCX-eGFP

    Plasmid

    #28310

    Depositor

    Joseph Loturco

    Article

    Manent et al Nat Med. 2009 Jan . 15(1):84-90.

    Insert

    Doublecortin (Dcx Rat)
    

    Use

    Cre/Lox

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2011

    Availability

    Academic Institutions and Nonprofits only

21. Cx26-sfGFP

    Plasmid

    #69026

    Purpose

    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx26 (Gjb2 Rat)
    

    Use

    Tags

    non-monomerized superfolder GFP (V206)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDNA GAL4 CREB 1-341 M1

    Plasmid

    #22973

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

    Insert

    CREB (Creb1 GAL4-fusion, Rat)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine of CREB

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHT-497

    Plasmid

    #87756

    Purpose

    Bacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)

    Depositor

    Yu-Wen Hwang

    Insert

    Gene coding for rat DYRK1A kinase domain (residues 1-497)
    

    Use

    Tags

    6X histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA polymerase promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEN_TT S6K (E389-deltaCT)

    Plasmid

    #58507

    Purpose

    Gateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pFSW Syt1-C2B IRES GFP

    Plasmid

    #133817

    Purpose

    Encodes the C2B domain of synaptotagmin 1 for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. alpha1Ddelta-G12-FRB

    Plasmid

    #87454

    Purpose

    rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    a1D short variants (Cacna1d Rat)
    

    Use

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    delete KYPAKNTTIALQMLERML

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRC/RSV-rat Agt

    Plasmid

    #122104

    Purpose

    Expression of angiotensinogen

    Depositor

    John Chan

    Article

    Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

    Insert

    angiotensinogen (Agt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. prNFL-P8R-EGFP

    Plasmid

    #132598

    Purpose

    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    Nefl (Nefl Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    23C>G; Pro8Arg

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. prNFL-N98S-EGFP

    Plasmid

    #132600

    Purpose

    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    Nefl (Nefl Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    293A>G; Asn98Ser

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge