We narrowed to 4,594 results for: DUR
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Plasmid#225439PurposeModule for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInsertHygR-MAR10-gemiGFP-MAR10
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Nova1-GFP
Plasmid#217033PurposeOverexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neuronsDepositorAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor
Plasmid#173208PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I-TOSI donor
UseCRISPRExpressionMammalianPromoterhPGK1Available SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-S1033A
Plasmid#211835PurposeVinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.DepositorInsertVinculinTS-S1033A (VCL Chicken, Synthetic)
UseLentiviralMutationmutated vinculin serine 1033 to alanine (S1033A),…PromoterCMVAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-S1033D
Plasmid#211893PurposeVinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.DepositorInsertVinculinTS-S1033D (VCL Chicken, Synthetic)
UseLentiviralMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinCS-S1033A
Plasmid#211894PurposeVinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.DepositorInsertVinculinCS-S1033A (VCL Chicken, Synthetic)
UseLentiviralMutationmutated vinculin serine 1033 to alanine (S1033A),…PromoterCMVAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinCS-S1033D
Plasmid#211895PurposeVinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.DepositorInsertVinculinCS-S1033D (VCL Chicken, Synthetic)
UseLentiviralMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA pCAGGS
Plasmid#206052Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain IIDepositorAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
186_pETcon_SARS2_XBB1-5
Plasmid#208086Purposeyeast surface display of the SARS-CoV-2 XBB.1.5 variant RBDDepositorInsertSARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVQA
Plasmid#197894PurposepCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))DepositorInsertHIV-1 3' end (HXB2: 9045 till poly-A(10)
ExpressionBacterialAvailable SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-Rab11
Plasmid#194336PurposeExpresses FLAG tagged Rab11 in mammalian cells and zebrafish embryosDepositorAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-Rab8
Plasmid#194335PurposeExpresses FLAG tagged Rab8 in mammalian cells and zebrafish embryosDepositorAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV puro PRDM14 ΔSET domain
Plasmid#193680PurposeConstitutive retroviral expression of PRDM14DepositorAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV puro PRDM14 ΔZinc finger domain
Plasmid#193679PurposeConstitutive retroviral expression of PRDM14DepositorAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1
Plasmid#184644PurposeRetroviral expression of BirA-mRap1DepositorInsertBirA-rap1
UseRetroviralTagsBirA FLAGExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-H3.3 (H3f3b) sgRNA
Plasmid#186931PurposeH3f3b sgRNA used for genomic targeting at H3f3b C-terminalDepositorAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1-I312R
Plasmid#184645PurposeRetroviral expression of BirA Rap1 I312RDepositorInsertBirA-rap1-I312R
UseRetroviralTagsBirA FLAGExpressionMammalianPromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-tetO-mCherry-attB53
Plasmid#183612PurposeRMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInserttetO-mCherry
UseRmcePromotertetOAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2604
Plasmid#175088PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationS644AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2602
Plasmid#175086PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationT424A, T438AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2603
Plasmid#175087PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationT504A, T508AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDON207 NSP3 K977Q
Plasmid#180057PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 NSP3 K977Q (ORF1ab SARS-CoV-2)
TagsMAC-tagExpressionMammalianMutationNSP3 K977QAvailable SinceJan. 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON207 NSP3 A890D
Plasmid#180069PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 NSP3 A890D (ORF1ab SARS-CoV-2)
TagsMAC-tagExpressionMammalianMutationNSP3 A890DAvailable SinceJan. 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON221 ORF3a Q57H
Plasmid#180072PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 ORF3a Q57H (ORF3a SARS-CoV-2)
TagsMAC-tagExpressionMammalianMutationORF3a Q57HAvailable SinceJan. 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON223 N S235F
Plasmid#180071PurposeSars-CoV-2 protein mutationDepositorAvailable SinceJan. 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON223 NSP5 K90R
Plasmid#180073PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 NSP5 K90R (ORF1ab SARS-CoV-2)
TagsMAC-tagExpressionMammalianMutationNSP5 K90RAvailable SinceJan. 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.U6gT28.3.hSyn.H2B.RFP
Plasmid#170370PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVPromoterhuman SynapsinAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA
Plasmid#178096PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + MTOR nick exon-44 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA
Plasmid#178099PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + MTOR nick intron-45 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA
Plasmid#178102PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA
Plasmid#178103PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA
Plasmid#178105PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick exon-44 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA
Plasmid#178106PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick intron-45 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA
Plasmid#178108PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA
Plasmid#178109PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC-TMPRSS11B
Plasmid#172421PurposeMAC-tagged gene expressionDepositorInsertTransmembrane protease serine 11B (TMPRSS11B Human)
TagsMAC-tagExpressionMammalianPromoterCMV promoterAvailable SinceNov. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA
Plasmid#173209PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#173211PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
UseCRISPR; Prime editing (pe3)ExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA
Plasmid#173212PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-loxP-canisNrg1
Plasmid#173849PurposeEncoding dog Nrg1 before Cre-mediated recombination.DepositorInsertA cDNA of canis Nrg1
ExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only