We narrowed to 1,197 results for: Aspa

1. pCR 2.1-TOPO Fzd6 N352A HA

   Plasmid

   #208365

   Purpose

   Extracellular HA-tagged Fzd6 N352A (mouse)

   Depositor

   Danelle Devenport, Maureen Cetera

   Article

   Cetera et al Development. 2023 Aug 25:dev.202078. doi

   Insert

   Fzd6 (Fzd6 Mouse)
   

   Use

   Cloning vector

   Tags

   HA

   Expression

   Mutation

   Asparagine 352 to Alanine

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1-zeo D476A ORF29

   Plasmid

   #200027

   Purpose

   Lentiviral vector for expression of KSHV ORF29 with D476A mutation

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   ORF29
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Aspartic acid 476 changed to Alanine

   Promoter

   CMV

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-RIPK3[S199D]-FLAG

   Plasmid

   #199315

   Purpose

   mutated phosphorylation site (kinase acitivity permanent active)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 199 to Aspartic Acid

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN HA-ATG13(Y118D)

   Plasmid

   #188638

   Purpose

   For expression of human ATG13(Y118D)

   Depositor

   Michael Lazarou

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed tyrosine 118 to aspartic acid

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBMN HA-ATG13(W50D)

   Plasmid

   #188639

   Purpose

   For expression of human ATG13(W50D)

   Depositor

   Michael Lazarou

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed tryptophan 50 to aspartic acid

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sandgrouse–insD

   Plasmid

   #191228

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   removed aspartic acid at 121 in the protein seque…

   Promoter

   PH and p10

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

   Plasmid

   #140455

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, leucine 624 to asparagine

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

   Plasmid

   #140456

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, alanine 634 to asparagine

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-HA-PfROM4 (G470A/G471N)

   Plasmid

   #154859

   Purpose

   Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfROM4 (PF3D7_0506900 Synthetic)
   

   Use

   Tags

   triple HA

   Expression

   Mammalian

   Mutation

   Glycine 470 changed to Alanine and Glycine 471 ch…

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid

    #154873

    Purpose

    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM1 (PF3D7_0506900 Synthetic)
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Alanine 144 changed to Glycine and Asparagine 145…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET151-GST-PP-RPN13 (19-132) K97D

    Plasmid

    #125824

    Purpose

    Expresses GST-RPN13 (19-132) K97D in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed lysine 97 to aspartate

    Promoter

    T7

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCu-GFP-Atg17(416) (I325D)

    Plasmid

    #121412

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 325 to Aspartic acid

    Promoter

    CUP1 promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCu-GFP-Atg17(416) (F317D)

    Plasmid

    #121411

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid

    Promoter

    CUP1 promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCu-GFP-Atg17(416) (L105D)

    Plasmid

    #121410

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 105 to Aspartic acid

    Promoter

    CUP1 promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGAD-Atg17(L313D I314K)

    Plasmid

    #121390

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 313 to Aspartic acid; Isoleucine 314 to L…

    Promoter

    ADH1 promoter

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCu-Atg17(416) (L105D)

    Plasmid

    #121405

    Purpose

    Expressing Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 105 to Aspartic acid

    Promoter

    CUP1 promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCu-Atg17(416) (F317D)

    Plasmid

    #121406

    Purpose

    Expressing Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid

    Promoter

    CUP1 promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCu-Atg17(416) (I325D)

    Plasmid

    #121407

    Purpose

    Expressing Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 325 to Aspartic acid

    Promoter

    CUP1 promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. N229A Lo-MetQ

    Plasmid

    #118268

    Purpose

    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Insert

    MetQ (N229A) (metQ E. coli)
    

    Use

    Tags

    10x His Tag and Enterokinase Cleavage Site

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 229 to Alanine (Ala)

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a - Ec-ClpX D144L

    Plasmid

    #111514

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Aspartatic acid 144 to Leucine

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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