We narrowed to 2,461 results for: T2A

1. LV anti-FITC-LaG16-R103A-T2A-BFP

   Plasmid

   #194935

   Purpose

   LV construct encoding R103A mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

   Plasmid

   #165030

   Purpose

   Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

   Depositor

   Yubin Zhou

   Article

   Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

   Insert

   LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2ADW-lox-mCherry-hyBRET-ERK-NLS

   Plasmid

   #170290

   Purpose

   For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   lox-mCherry-lox-hyBRET-ERK-NLS
   

   Expression

   Mammalian

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

   Plasmid

   #178585

   Purpose

   Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   DLX2 (DLX2 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

   Plasmid

   #176892

   Purpose

   Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Article

   Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

   Insert

   moxGFP-P2A-T2A-c-Myc
   

   Use

   Transposon

   Tags

   Myc and moxGFP

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

   Plasmid

   #204975

   Purpose

   Co-express EGFP and SARS-CoV-2 ORF6

   Depositor

   Tim Mitchison

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   Open Reading Frame 6 (ORF6 SARS-CoV-2)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTY152 EGFP-T2A-ORF6 SARS-CoV-1

   Plasmid

   #204974

   Purpose

   Co-express EGFP and SARS-CoV-1 ORF6

   Depositor

   Tim Mitchison

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   Open Reading Frame 6 (ORF6 SARS-CoV-1)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-mir144 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164791

   Purpose

   Express miR-144 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144 (MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

   Plasmid

   #110830

   Purpose

   Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   AZGP1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   T2A and dTomato

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid

    #117070

    Purpose

    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

    Depositor

    Kim Failor

    Inserts

    Cas9
    SacB
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

    Plasmid

    #31478

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 K227M T2A BFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    F13Y, S111Y, K227M = Y2 nickase "reo" …

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid

    #185699

    Purpose

    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter

    Depositor

    Lynette Lim

    Inserts

    Tdtomato,
    Synaptophysin-EGFP
    

    Use

    Cre/Lox and Retroviral

    Tags

    EGFP and T2A

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78306

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADfluw-Hsp70
    

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid

    #85844

    Purpose

    Genetically encoded voltage sensor ArcLight- codon optimized

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    ArcLightCo-Q239
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    hSyn1

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102891

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

    Plasmid

    #72258

    Purpose

    Express FLPe, copGFP and Puromycin resistance gene under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-copGFp-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available Since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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