We narrowed to 159,238 results for: addgene

1. pLV CMV IGF1-FLAG

   Plasmid

   #175170

   Purpose

   Lentiviral expression of human Igf1-FLAG

   Depositor

   Larry Gerace

   Insert

   Igf1 (IGF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SND1 (SND1A-c003)

   Plasmid

   #98242

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SND1A (SND1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00567:TUDOR 677-799

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6VAH

   Plasmid

   #162273

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   TEAD2 (TEAD2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SETD1A

   Plasmid

   #32868

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETD1A (SETD1A Human)
   

   Use

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. integrin alpha9 pBabe puro

   Plasmid

   #13604

   Depositor

   Dean Sheppard

   Insert

   integrin alpha 9 (ITGA9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   contains mature ITGA9 (aa30-1035 compared to NP_0…

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

6. XLone-Puro NFIL3 P2A eGFP NLS

   Plasmid

   #140026

   Purpose

   Tunable and temporal expression control of NFIL3 and nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-FLAG-mMdm2-IRES-GFP

   Plasmid

   #66955

   Purpose

   Retroviral vector to express a FLAG-tagged mouse Mdm2

   Depositor

   Martine Roussel

   Insert

   Mdm2 (Mdm2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. AAV pCAG-FLEX-mRuby3-WPRE

   Plasmid

   #99279

   Purpose

   Can be used to generate AAV virus that will express mRuby3 in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KDM4D

   Plasmid

   #73247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KDM4D (KDM4D Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam02375:JmjN 18-64, pfam02373:JmjC 182-295

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTEAD1.1.0-gDNA

    Plasmid

    #112425

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

    Depositor

    Kevin White

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV Cbx1-V5

    Plasmid

    #175106

    Purpose

    Lentiviral expression of mouse Cbx1-V5

    Depositor

    Larry Gerace

    Insert

    Cbx1 (Cbx1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRMT10C (RG9MTD1A-c113)

    Plasmid

    #110248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RG9MTD1 (TRMT10C Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-ABEmax-SpG

    Plasmid

    #195278

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

    Depositor

    Petros Patsali, Carsten Lederer

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AD-003

    Plasmid

    #25502

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AD-003 (NTMT1 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. ARF1

    Plasmid

    #28168

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

16. 4QPP

    Plasmid

    #61964

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Expression

    Mutation

    See comments

    Promoter

    Polyhedrin

    Available since

    Sept. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid

    #174902

    Purpose

    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Y306A, Y384F

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PKD2 (PKD2A-c034)

    Plasmid

    #98226

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PKD2A (PKD2 Human)
    

    Use

    Baculovirus

    Tags

    TEV-His10-Flag

    Expression

    Mutation

    pfam00520:Ion_trans 475-680

    Promoter

    polyhedrin

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pINDUCER20-FLAG-hSRF (#799)

    Plasmid

    #78345

    Purpose

    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pELF2.1.0-gDNA

    Plasmid

    #132454

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ELF2 (ELF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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