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pUGP1.1

Plasmid

#196615

Purpose

Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)

Use

Tags

AID(71-114) and mCherry

Expression

Yeast

Mutation

Promoter

Native promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pT22gcfUbm5En25EE4PCh(#552)

Plasmid

#184092

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

5myc-chkEn2(5E)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm4Eng2bKRPC(#493)

Plasmid

#184091

Purpose

cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

4xMyc

Expression

Mutation

ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF713

Plasmid

#121665

Purpose

U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-AtAEP2

Plasmid

#159529

Purpose

Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

Depositor

Joshua Mylne

Article

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Arabidopsis thaliana asparaginyl endopeptidase 2

Use

Tags

Six-Histidine tag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCF730

Plasmid

#121668

Purpose

EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2r

Plasmid

#120199

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2

Plasmid

#120198

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTC364

Plasmid

#91213

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC365

Plasmid

#91214

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC366

Plasmid

#91215

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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CRISPRi-CENPB-g2

Plasmid

#120210

Purpose

CENPB CRISPRi guide RNA 2

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB CRISPRi guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-YU2gp160

Plasmid

#100927

Purpose

Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (YU2) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMaster12

Plasmid

#58527

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
Myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
NANOG (NANOG Human)
IRES
NR5A2 (NR5A2 Human)
miR302/367 (MIR367, MIR302A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMaster3

Plasmid

#58526

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 )
myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
Nanog (NANOG Human)
IRES
NR5A2 (NR5A2 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 WT

Plasmid

#123075

Purpose

Gateway entry clone encoding human MAP1LC3B2 wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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C413-E19: attB4-mPol2p>-attB1r

Plasmid

#162971

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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