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pUC57-Amp_CRISPIE donor B3

Plasmid

#172844

Purpose

CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mTurquoise2

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

Plasmid

#60492

Purpose

Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

3xnls-SGFP2(T65G)
SGFP2-3xNES

Tags

SGFP2 and SGFP2(T65G)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5_NLS-Halo-eGFP-BioID

Plasmid

#218448

Purpose

expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BirA

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFU-MVIIA-PE

Plasmid

#24144

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Expression

Mammalian

Available Since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6017nes

Plasmid

#108655

Purpose

A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-PKA-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pVRb01_syn20

Plasmid

#49704

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf01_syn20

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-6085x

Plasmid

#108653

Purpose

A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

PicchuEV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.11

Plasmid

#191921

Purpose

Encodes a GFP design, sf:mid.11

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.11

Tags

His Tag

Expression

Bacterial

Mutation

Q69M Y145F T167I L220V V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.7

Plasmid

#191933

Purpose

Encodes a GFP design, sf:mid.7

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.7

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145F V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.6

Plasmid

#191912

Purpose

Encodes a GFP design, acid.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.6

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72A Y145M T167V H181L

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:acid.3

Plasmid

#191926

Purpose

Encodes a GFP design, sf:acid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:acid.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69L S72A T108V Y145M V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.7

Plasmid

#191900

Purpose

Encodes a GFP design, mid.7

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.7

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145F V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-jRCaMP1b

Plasmid

#191468

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

jRCaMP1b

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-SYN-GFP-BGHpA

Plasmid

#190229

Purpose

Expresses EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pVRb22_up1147

Plasmid

#49716

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf22_up1147

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb03_up1198

Plasmid

#49706

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf03_up1198

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-PUbEGFP-NLS

Plasmid

#176681

Purpose

Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EGFP

Expression

Insect

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFU-cMVIIA-PE

Plasmid

#24147

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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