We narrowed to 15,910 results for: grna

Showing: 9581 - 9600 of 15910 results

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pXPR_501

Plasmid

#96922

Purpose

for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

Depositor

John Doench, David Root

Publication

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMM710

Plasmid

#68893

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Immune checkpoint (library 2) Backbone

Plasmid

#192363

Purpose

Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

Depositor

Lijia Ma

Publication

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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T cell activation (library 1) Backbone

Plasmid

#192364

Purpose

Plasmid vector for in vitro Jurkat activation screening (for immune library)

Depositor

Lijia Ma

Publication

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330-PITCh-GOLGA2

Plasmid

#207791

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_p65_hsf1

Plasmid

#79374

Purpose

Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAC1816_pCR8-gSMN2-DN4

Plasmid

#118649

Purpose

Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ131D

Plasmid

#69287

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAS088

Plasmid

#211502

Purpose

AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

Depositor

Randall Platt

Publication

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Type

Empty backbone

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mU6-sgNT-hU6-sgLkb1_1st

Plasmid

#177221

Purpose

Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNT1/sgLkb1_1st

Use

Lentiviral

Promoter

mU6/hU6

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgNT-hU6-sgLkb1_2nd

Plasmid

#177223

Purpose

Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNT1/sgLkb1_2nd

Use

Lentiviral

Promoter

mU6/hU6

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgLkb1_2nd-hU6-sgNT

Plasmid

#177222

Purpose

Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgLkb1_2nd/sgNT1

Use

Lentiviral

Promoter

mU6/hU6

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mU6-sgSik1_2nd-hU6-sgSik3_1st

Plasmid

#177218

Purpose

Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgSik1_2nd/sgSik3_1st

Use

Lentiviral

Promoter

mU6/hU6

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mU6-sgSik1_1st-hU6-sgSik3_2nd

Plasmid

#177217

Purpose

Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgSik1_1st/sgSik3_2nd

Use

Lentiviral

Promoter

mU6/hU6

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mU6-sgSik1_1st-hU6-sgSik3_1st

Plasmid

#177216

Purpose

Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgSik1_1st/sgSik3_1st

Use

Lentiviral

Promoter

mU6/hU6

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mU6-sgSik3_2nd-hU6-sgNT

Plasmid

#177215

Purpose

Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgSik3_2nd/sgNT1

Use

Lentiviral

Promoter

mU6/hU6

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mU6-sgSik3_1st-hU6-sgNT

Plasmid

#177214

Purpose

Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgSik3_1st/sgNT1

Use

Lentiviral

Promoter

mU6/hU6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-dCasRx-RBM25-IRES-BleoR

Plasmid

#221002

Purpose

Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

Depositor

Benjamin Blencowe, Mikko Taipale

Publication

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

dCasRx-RBM25-IRES-BleoR

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Promoter

CAG

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p{CFD4-3xP3::DsRed}

Plasmid

#86864

Purpose

Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAN-NOR

Plasmid

#62306

Purpose

synthetic circuit: NOR gate

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PPhlF and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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