We narrowed to 10,139 results for: yeast

1. pDIV495

   Plasmid

   #177702

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ash1-5p

   Plasmid

   #169712

   Purpose

   Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

   Depositor

   Tanja Mittag

   Article

   Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

   Insert

   Ash1 (ASH1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation including encoding amino acids 420-480…

   Promoter

   T7

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPS1-Venus

   Plasmid

   #133118

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pYPS1-YFP
   

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMK2-Venus

   Plasmid

   #133119

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pCMK2-YFP
   

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGYP7-Venus

   Plasmid

   #133120

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pGYP7-YFP
   

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. YCplac33-NAT_NDT1

   Plasmid

   #140593

   Purpose

   Express NDT1 in S. cerevisiae

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   NDT1 promoter and 3'UTR

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCu-GFP-Atg17(416) (I325D)

   Plasmid

   #121412

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Isoleucine 325 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCu-GFP-Atg17(416) (F317D)

   Plasmid

   #121411

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Phenylalanine 317 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCu-GFP-Atg17(416) (L105D)

   Plasmid

   #121410

   Purpose

   Expressing GFP-Atg17

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   CUP1p-GFP-ATG17 ORF-CYC1 terminator
   

   Expression

   Yeast

   Mutation

   Leucine 105 to Aspartic acid

   Promoter

   CUP1 promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCu-Atg17(416) (L105D)

    Plasmid

    #121405

    Purpose

    Expressing Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-ATG17 ORF-CYC1 terminator
    

    Expression

    Yeast

    Mutation

    Leucine 105 to Aspartic acid

    Promoter

    CUP1 promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZ-pre-Ost1-pro-alphaf-Btl2

    Plasmid

    #117665

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-pro-alphaf-Btl2
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW106

    Plasmid

    #111138

    Purpose

    For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

    Depositor

    Alexander Varshavsky

    Article

    Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

    Insert

    MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
    

    Tags

    3x Flag

    Expression

    Yeast

    Mutation

    Leu2 deletion

    Promoter

    Gal1

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 719-2µ-minHIS3

    Plasmid

    #40610

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast, Synthetic)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-SpELL pNMT-TOPO

    Plasmid

    #15315

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Tags

    FLAG

    Expression

    Yeast

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. FLAG-SpEAF pNMT-TOPO

    Plasmid

    #15316

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    ELL associated factor (eaf1 Fission Yeast)
    

    Tags

    FLAG

    Expression

    Yeast

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pPICZ:hTRAAK(W262S)

    Plasmid

    #130670

    Purpose

    P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    KCNK4 (KCNK4 Human)
    

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    N104Q, N108Q, W262S

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available Since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pTH727-CEN-RLuc/staCFLuc

    Plasmid

    #38211

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCLASP_mScarlet_CLASP_RGS2membrane

    Plasmid

    #133086

    Purpose

    Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    mScarlet-CLASP
    

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

    Plasmid

    #176395

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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