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pHR_PGK_antiCD19_synNotch_Gal4VP64

Plasmid

#79125

Purpose

Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

antiCD19_synNotch_Gal4VP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human 7SK

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-tmpknot-GG-acceptor

Plasmid

#174039

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMD2.G-VSV-G-mut

Plasmid

#196508

Purpose

VSV-G mutant (K47Q, R354A)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

VSV-G

Tags

NA

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pKM495 (Flag-Das+4) Zeo

Plasmid

#109284

Purpose

ORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-DAS+4

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pKM444

Plasmid

#108319

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor

Expression

Bacterial

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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Marathon-RT for Split-PE (pEH59)

Plasmid

#190108

Purpose

Expresses Marathon-RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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gag-P3-pol

Plasmid

#211374

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-P3-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_pGK_LaG17_synNotch_Gal4VP64

Plasmid

#79127

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17_synNotch_Gal4DBDVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mTagBFP2

Plasmid

#113725

Purpose

Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

Depositor

Vanessa LaPointe

Article

Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

Insert

mTagBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRC0569 (parts entry vector)

Plasmid

#189966

Purpose

Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

BBa-J23100

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-biRhoBAST

Plasmid

#196348

Purpose

Expression of mEGFP mRNA with biRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-biRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor MCS Rosa26

Plasmid

#37200

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Inserts

Rosa26 left targeting arm
MCS
Rosa26 right targeting arm

Use

Genomic targeting - zinc finger

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-ctrl

Plasmid

#85741

Purpose

shRNA ctrl

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA ctrl

Use

AAV

Tags

EYFP

Available Since

April 22, 2017

Availability

Academic Institutions and Nonprofits only

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HBA1 full 3'UTR-T4 td downstream intron

Plasmid

#189974

Purpose

Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

HBA1 full 3'UTR-T4 td downstream intron

Use

Cloning

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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gag-COM-pol

Plasmid

#211373

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-COM-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBT114

Plasmid

#138523

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

phage genes

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltNLS/NES-40-mCherry

Plasmid

#234066

Purpose

Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltNLS/NES-40-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-9-ZmUbi-11

Plasmid

#197777

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmUbi terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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