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pDV044

Plasmid

#191824

Purpose

Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

Depositor

Jim Roberts

Article

Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi2-6

Plasmid

#197706

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi2 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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gag-PE

Plasmid

#211371

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-PE

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023

Plasmid

#90324

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FAH-rtTA3G

Plasmid

#120309

Purpose

An AAV vector that expresses rtTA3G under the c-fos promoter

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

rtTA3G

Use

AAV

Promoter

c-fos

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pV1093

Plasmid

#111428

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMO819

Plasmid

#232072

Purpose

Expresses Cytosine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Cytosine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMO1170

Plasmid

#232073

Purpose

Expresses Adenine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Adenine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pChimera

Plasmid

#61476

Purpose

Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

U6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

U6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Expression

Mammalian

Promoter

MiniCMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cerebody

Plasmid

#236738

Purpose

Protein overexpression of cerebody

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

cerebody

Tags

His, HA, FLAG

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-C1-AKT-PH

Plasmid

#21218

Depositor

Tobias Meyer

Article

Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Insert

AKT-PH

Tags

GFP

Expression

Mammalian

Mutation

PH domain of AKT. (Lab plasmid WC 0002).

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-EGFP

Plasmid

#122848

Purpose

N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_SFFV_LaG17_synNotch_TetRVP64

Plasmid

#79128

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17 GFP nanobody synNotch TetRVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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gag-MCP-pol

Plasmid

#211370

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-MCP-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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