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pJYDN2p

Plasmid

#162455

Purpose

Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-DnbALFA (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA nanobody and HA and myc

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKCepsilon.RNAi

Plasmid

#10798

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC epsilon RNAi (PRKCE Human)

Use

RNAi

Expression

Mammalian

Available Since

April 7, 2006

Availability

Academic Institutions and Nonprofits only
p8.9 NdeltaSB

Plasmid

#132929

Purpose

Minimal HIV-1 packaging plasmid for gag and pol expression

Depositor

Jeremy Luban

Article

Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

Insert

gag and pol

Use

Lentiviral

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL hygro 12S E1A

Plasmid

#18748

Depositor

Scott Lowe

Article

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pHG4

Plasmid

#188252

Purpose

Integrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selection

Depositor

Hong Gao, Maggie Smith

Article

Gao et al BMC Biotechnol. 2019 Jun 4;19(1):32. doi

Type

Empty backbone

Expression

Bacterial

Promoter

act1p/actII-orf4

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBbB2k-GFP

Plasmid

#35345

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-RandE3-P2A-GFP

Plasmid

#236179

Purpose

AAV version of Tet inducible control plasmid

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

RandE3-P2A-GFP

Use

AAV

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXI Neo DsRed-LC3-GFP

Plasmid

#31183

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

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B2380_BTB-IDR

Plasmid

#215098

Purpose

Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR) in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-mCherry-FUS(IDR)

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSaI14

Plasmid

#8614

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI14

Expression

Bacterial

Mutation

N terminal threonine changed to alanine

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
PD23

Plasmid

#164536

Purpose

Expresses SmoM2-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened W539L (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

W539L

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-ALFANb-GB1-CAAX-WPRE

Plasmid

#231599

Purpose

Express ALFANb-CAAX

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

ALFANb-CAAX

Use

Lentiviral

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pSaI20

Plasmid

#8615

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI20

Expression

Bacterial

Mutation

N terminal threnine changed to alanine

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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ptdTomato-SMCR8

Plasmid

#83438

Purpose

Expresses SMCR8-tdTomato in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

Human SMCR8 (SMCR8 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJJS001

Plasmid

#188324

Purpose

PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::GFP::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCPP5372

Plasmid

#128733

Purpose

Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

Depositor

Alan Collmer

Article

Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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GFP E17TAG

Plasmid

#68295

Purpose

E17TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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GP735

Plasmid

#164537

Purpose

Expresses SmoM2-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened W539L (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

W539L

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHA-PUMA

Plasmid

#16588

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

PUMA (BBC3 Human)

Tags

2x HA

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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