We narrowed to 88,258 results for: MAL

1. pLenti GW-NL-myc

   Plasmid

   #113455

   Purpose

   Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   NanoLuc-cmyc

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT15542_nCBE-SuperFi-Cas9

   Plasmid

   #184372

   Purpose

   Mammalian expression of SuperFi-Cas9 CBE base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nCBE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT15544_nABE-SuperFi-Cas9

   Plasmid

   #184374

   Purpose

   Mammalian expression of SuperFi-Cas9 ABE7 base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nABE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   FLAG, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330 L-FKBP-Cas9

   Plasmid

   #162728

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

   Plasmid

   #108773

   Purpose

   Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   VCre recombinase fragment N-terminus to aa269
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

   Plasmid

   #108774

   Purpose

   Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   VCre recombinase fragment aa270 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IP/SECFP-mATG5

   Plasmid

   #58744

   Purpose

   Expresses /SECFP-ATG5 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Use

   Retroviral

   Tags

   SECFP

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

   Plasmid

   #176838

   Purpose

   To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   sgPDX1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

   Plasmid

   #159894

   Purpose

   AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

   Insert

   NES-tdTomato-LINuS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α promoter

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMinDis.X513-iNicSnFR3b-(V7)-PM

    Plasmid

    #125124

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)-PM
    

    Use

    Tags

    Ig-K-Chain and membrane anchoring protein-PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClneoMyc MOB1 T12A

    Plasmid

    #47936

    Purpose

    expresses Myc tagged human MOB1 containing T12A mutation

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T12A

    Promoter

    CMV

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCOCK CM-R03

    Plasmid

    #46567

    Purpose

    Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).

    Depositor

    Clifford Wang

    Article

    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

    Insert

    CMV mutant promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Mutant version of CMV promoter (refer to Table 1 …

    Promoter

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pJZC77

    Plasmid

    #62338

    Purpose

    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. DPYS-FLAG

    Plasmid

    #61618

    Purpose

    To study the effect of DPYS activity on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    dihydropyrimidinase (DPYS Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CRY-tTA (B702)

    Plasmid

    #92032

    Purpose

    Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    tTA2 (TetR fused to VP64)

    Expression

    Mammalian

    Mutation

    NLS on CRY2 is mutated

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDual-eGFP(Stop66)

    Plasmid

    #63218

    Purpose

    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Stop66)
    

    Use

    Lentiviral

    Tags

    T7 and his6

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. D998-EGFR-ET1

    Plasmid

    #44186

    Depositor

    Timothy Springer

    Article

    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

    Insert

    D998-EGFR-ET1 (EGFR Human)
    

    Use

    Tags

    3C cleavge site, protein C peptide tag, his6, SBP…

    Expression

    Mammalian

    Mutation

    EGFR amino acids 1−998

    Promoter

    CVM

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCOCK CMVwt

    Plasmid

    #46559

    Purpose

    Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

    Insert

    CMV wt promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Our version has a G at base 724 (reference has C)

    Promoter

    CMV

    Available Since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJZC73

    Plasmid

    #62341

    Purpose

    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    COM-KRAB
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDual-eGFP(W66)

    Plasmid

    #63217

    Purpose

    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(W66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only