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pOGG050

Plasmid

#113999

Purpose

SC module. Thermostable beta-galactosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

celB

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG083

Plasmid

#114001

Purpose

SC module. Beta-glucosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

gusA

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hR

Plasmid

#60997

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pLIC.B3.AF2373

Plasmid

#11529

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Nov 25. 354(2):289-303.

Insert

NAD kinase (AF2373 A. fulgidus)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pDest490-OPN-10 kDa

Plasmid

#17595

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-10 kDa (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 499–630

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pDest490-OPN-CT

Plasmid

#17593

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-CT (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 631–942

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pOGG039

Plasmid

#113996

Purpose

SC module. Terminator for T7RNAP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Terminator for T7RNAP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-4a

Plasmid

#61314

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and TRX-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
BtuCD

Plasmid

#92208

Purpose

Expression of BtuCD in E. coli

Depositor

Douglas Rees

Article

Locher et al Science. 2002 May 10;296(5570):1091-8.

Inserts

btuC (btuC )
btuD (btuD )

Tags

10x Histidine Tag and Enterokinase Cleavage Site

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hG

Plasmid

#60994

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear EGFP

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EGFP

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hC

Plasmid

#60993

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear cerulean

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

Cerulean

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pCri-6a

Plasmid

#61315

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

GST-tag and His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pBad_crtEBI_27

Plasmid

#63806

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-12b

Plasmid

#61334

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-9a-Strep

Plasmid

#61341

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

Strep-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pLXMA _RK2 L1A

Plasmid

#153348

Purpose

Expression vector for plants with low-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-DR RecR

Plasmid

#12617

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II

Plasmid

#120315

Purpose

Septin 11 isoform II mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp9-3

Plasmid

#73854

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pMXLP_pFa

Plasmid

#114239

Purpose

POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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