We narrowed to 53,437 results for: plasmid

1. pTXB3-Flag PLK1 T210D

   Plasmid

   #68272

   Purpose

   bacterial expression of Plk1 T210D mutant

   Depositor

   Stefano Ferrari

   Insert

   PLK1 (PLK1 Human)
   

   Use

   Tags

   Flag and Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   T210D

   Promoter

   T7

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLIX403-EYFP-ccdB-EBFP2

   Plasmid

   #158553

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.3 CAS9 IRES-Dsred

   Plasmid

   #129444

   Purpose

   expression of FnCas9 and DsRed

   Depositor

   Oskar Laur

   Insert

   FnCas9 IRES-Dsred
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHXPL

   Plasmid

   #17667

   Depositor

   Cecilia Lo

   Insert

   Cx43 promoter (Gja1 Mouse)
   

   Use

   Tags

   LacZ

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS3ndd

   Plasmid

   #97354

   Purpose

   Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant

   Depositor

   Jamorn Somana

   Insert

   Nucleoid Disruptive Domain
   

   Use

   Cloning

   Tags

   Expression

   Bacterial

   Mutation

   changed methionine 1 to isoleucine

   Promoter

   E. coli lacZ promoter

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-DmUPF3-V5His6_D

   Plasmid

   #146098

   Purpose

   Insect Expression of DmUPF3

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF3 (Upf3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent and non silent mutation P305S compared t…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nup54-mEGFP494(flex1)

   Plasmid

   #163430

   Purpose

   Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 6 amino acids at the carboxyl end of the mEGFP

   Depositor

   Sanford Simon

   Insert

   Nup54 (NUP54 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nup54-mEGFP494(1)

   Plasmid

   #163427

   Purpose

   Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of the mEGFP

   Depositor

   Sanford Simon

   Insert

   Nup54 (NUP54 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-XL4 FLAG-TM9SF4-Fc

   Plasmid

   #115770

   Purpose

   Expression of the extracellular domain of TM9SF4 with N-terminal FLAG tag and C-terminal hIgG tag.

   Depositor

   Davide Comoletti

   Insert

   TM9SF4 (TM9SF4 Human)
   

   Use

   Tags

   FLAG and hIgG/Fc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-mxLOX1

    Plasmid

    #104975

    Purpose

    Biosynthesis of oxylipins by microbial enzymes

    Depositor

    Deokkun Oh

    Insert

    MXAN_1745 (MXAN_1745 Myxococcus xanthus)
    

    Use

    Tags

    6 histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-mxLOX2

    Plasmid

    #104976

    Purpose

    Biosynthesis of oxylipins by microbial enzymes

    Depositor

    Deokkun Oh

    Insert

    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    

    Use

    Tags

    6 histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXB7-pMAL

    Plasmid

    #20996

    Depositor

    Corey Largman

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Tags

    mal

    Expression

    Bacterial

    Mutation

    Coding region only

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCI-ING5

    Plasmid

    #79071

    Purpose

    Expression of ING5 in mammalian cells

    Depositor

    Karl Riabowol

    Insert

    ING5 (ING5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pLenti6.3_HES2

    Plasmid

    #124070

    Purpose

    HES2 with normal stop codon

    Depositor

    J. Chad Brenner

    Insert

    HES2 (HES2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. SRS-MTBD(19/19), AB(monomer), ux

    Plasmid

    #22389

    Depositor

    Ron Vale

    Insert

    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescript KS(-) otk

    Plasmid

    #17586

    Depositor

    Denise Montell

    Insert

    otk (otk Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

17. MEIS1B-pVP16

    Plasmid

    #21020

    Depositor

    Corey Largman

    Insert

    MEIS1B (Meis1 Mouse)
    

    Use

    Tags

    VP16-AD

    Expression

    Mammalian

    Mutation

    VP16 activation domain fused to N-terminal

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid

    #112490

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K599R

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. XE181 rat Frizzled-1-myc-pCD

    Plasmid

    #16819

    Depositor

    Randall Moon

    Insert

    frizzled-1 (Fzd1 Rat)
    

    Use

    Xenopus expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

20. Rab5-L38R-mCherry

    Plasmid

    #102785

    Purpose

    Point mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308D

    Depositor

    Donna Webb

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Leucine 38 changed to Arginine

    Promoter

    CMV

    Available Since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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