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Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCT-L7.5.1

Plasmid

#42900

Depositor

Dane Wittrup

Article

Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

Insert

L7.5.1

Tags

HA and c-myc

Expression

Yeast

Promoter

Gal1-10

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-Myc-TRIP12

Plasmid

#241813

Purpose

Expresses Myc-tagged TRIP12 in mammalian cells.

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

TRIP12 (TRIP12 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pRP-B-catenin-V5-LgBiT

Plasmid

#241816

Purpose

Expresses V5-tagged and LgBiT-tagged B-catenin for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

B-catenin

Tags

LgBiT and V5

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 PDZD8-WT

Plasmid

#227849

Purpose

To express in E. coli PDZD8-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDZD8 (PDZD8 Human)

Tags

GST

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC EPHA2

Plasmid

#227879

Purpose

Used for transfection of cells for expression of C-MYC EPHA2

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

EPHA2 (EPHA2 Human)

Tags

MYC

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBOBI C-GFP PDZD8

Plasmid

#227823

Purpose

To amplify the virus that expresses C-GFP PDZD8

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDZD8 (PDZD8 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en15FnCas9

Plasmid

#201948

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en15FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1603H on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

Plasmid

#209086

Purpose

Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Tags

AviTag

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

Plasmid

#209085

Purpose

Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)
BirA

Expression

Mammalian

Mutation

human codon optimized

Promoter

TRE4G and hSyn

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pExp-RBD-CHis

Plasmid

#195002

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

Depositor

Marko Hyvönen

Article

Fischer et al Nat Biotechnol. 2025 Jun;43(6):960-970.

Insert

RBD (S SARS-CoV-2)

Tags

His8

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti AID-BEmax

Plasmid

#157950

Purpose

Lentiviral expression of AID-BEmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti AID-BEmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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KRAS-A146T

Plasmid

#154426

Purpose

A146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET30-2-PHGDH-C234S

Plasmid

#107724

Purpose

Expression of PHGDH C234S in bacteria

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

PHGDH (PHGDH Human)

Expression

Bacterial

Mutation

C234S

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISPAFabH

Plasmid

#97018

Purpose

Expressing Pseudomonas aeruginosa FabH protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

3-Oxoacyl-[acyl-carrier-protein] synthase III

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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HCT47_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA

Plasmid

#233564

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Alpha

Plasmid

#177961

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )

Expression

Mammalian

Mutation

del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

Plasmid

#159127

Purpose

expresses FBXO22 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

FBXO22 (FBXO22 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0915 (CRISPR with target 3)

Plasmid

#130643

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(target-3)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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