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Anti-Homer1L/S [L113/130R-1]

Plasmid

#206687

Purpose

Mammalian Expression Plasmid of anti-Homer1L/S (Mouse). Derived from hybridoma L113/130-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Homer1L/S (Mus musculus) recombinant Mouse monoclonal antibody (Homer1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-HCN3 [N141/21R]

Plasmid

#206614

Purpose

Mammalian Expression Plasmid of anti-HCN3 (Mouse). Derived from hybridoma N141/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN3 (Mus musculus) recombinant Mouse monoclonal antibody (Hcn3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

Plasmid

#218654

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3 (STOP)

Plasmid

#218638

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msPlekha8 (msFapp2) g1 lentiCRISPRv2-opti

Plasmid

#218650

Purpose

Knockout vector for mouse Plekha8 (Fapp2)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha8 (Plekha8 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msPlekha3 (msFapp1) g2 lentiCRISPRv2-opti

Plasmid

#218649

Purpose

Knockout vector for mouse Plekha3 (Fapp1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha3 (Plekha3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msPlekha3 (msFapp1) g1 lentiCRISPRv2-opti

Plasmid

#218648

Purpose

Knockout vector for mouse Plekha3 (Fapp1)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha3 (Plekha3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msPlekha8 (msFapp2) g2 lentiCRISPRv2-opti

Plasmid

#218651

Purpose

Knockout vector for mouse Plekha8 (Fapp2)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Plekha8 (Plekha8 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msHook2 g2 lentiCRISPRv2-mCherry

Plasmid

#218653

Purpose

Knockout vector for mouse Hook2

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Hook2 (Hook2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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Msi1_2xRRM_pGEX

Plasmid

#215704

Purpose

Recombinant expression of mouse Msi1 with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

Musashi-1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Crym-BioID2-BioID2

Plasmid

#200070

Purpose

To study u-crystallin protein interactors

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-Crym-BioID2-BioID2

Use

AAV and Mouse Targeting

Tags

HA-tag

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Crym-eGFP

Plasmid

#200080

Purpose

To study u-crystallin protein interactors, control plasmid

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-Crym-eGFP

Use

AAV and Mouse Targeting

Tags

eGFP

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138E

Plasmid

#208287

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138E

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mustard Weed, Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Glu

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138A

Plasmid

#208288

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138A

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin (Cry2, PRF1 Mustard Weed, Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Ala

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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