4,178 results

1. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2 neo

   Plasmid

   #98292

   Purpose

   Variant of lentiCRISPRv2 that confers G418 resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF-1a

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mcherry-PH

   Plasmid

   #36075

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   PH domain of PLCdelta1 (PLCD1 Human)
   

   Tags

   mcherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 GFP shRNA

   Plasmid

   #30323

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   GFP shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. JAK2 (V617F)-pcw107-V5

   Plasmid

   #64610

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   V617F

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9(1.1)

   Plasmid

   #71814

   Purpose

   Expresses high specificity SpCas9. Px330-like plasmid.

   Depositor

   Feng Zhang

   Article

   Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

   Has Service

   Cloning Grade DNA

   Insert

   enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBa-LSS-GFP-LDLR wt

   Plasmid

   #98184

   Purpose

   Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

   Depositor

   Gary Banker, Marvin Bentley

   Article

   Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

   Insert

   LDLR (LDLR Human)
   

   Tags

   GFP and LDLR signal sequence, N-term of GFP so GF…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta Actin

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-G3BP1-WT

   Plasmid

   #135997

   Purpose

   WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mfn2-Myc

    Plasmid

    #23213

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn2 (Mfn2 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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