4,076 results

1. pTD103luxI_sfGFP

   Plasmid

   #48885

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   LuxI, sfGFP, and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-iCAP-PHP.S

   Plasmid

   #103006

   Purpose

   non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   Synthetic construct isolate AAV-PHP.S VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3-EF1a-c-Met

   Plasmid

   #31784

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 EYFP H148Q/I152L

   Plasmid

   #25872

   Depositor

   Peter Haggie

   Article

   Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

   Insert

   EYFP H148Q/I152L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP from pEYFP (Clontech) with H148Q and I152L m…

   Promoter

   Available since

   Aug. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pR26 CAG/GFP Dest

   Plasmid

   #74281

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330 Pten

   Plasmid

   #59909

   Purpose

   pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ptfLC3

   Plasmid

   #21074

   Purpose

   Mammalian expression of rat LC3 fused to mRFP and EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 HA eIF4GI (1-1599)

   Plasmid

   #45640

   Depositor

   Nahum Sonenberg

   Article

   Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

   Insert

   eIF4GI transcript variant 2 (EIF4G1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

    Plasmid

    #40640

    Depositor

    Brenda Lilly

    Article

    Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

    Insert

    NICD3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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