19,762 results

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

Plasmid

#42335

Purpose

A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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TATA-GFP

Plasmid

#110833

Purpose

negative control

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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eGFPC1 FKBP

Plasmid

#67529

Purpose

Expresses GFP fused FKBP

Depositor

Stephen Tait

Article

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Insert

FKBP

Tags

eGFP

Expression

Mammalian

Mutation

F36V

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pQE30-hPolB

Plasmid

#70761

Purpose

bacterial expression of human DNA Polymerase beta (POLB)

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

POLB (POLB Human)

Tags

6x His

Expression

Bacterial

Mutation

R333L

Promoter

T5/lac operon

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag

Plasmid

#51246

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only
pCAG/GFP

Plasmid

#139980

Purpose

expresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Green fluorescent protein

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1039

Plasmid

#119239

Purpose

expresses Tn7 transposase

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

tnsABCD

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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3xERRE-luciferase

Plasmid

#37851

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE upstream of luciferase

Use

Luciferase

Promoter

SV40

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B WT

Plasmid

#175025

Purpose

Expresses WIPI2B fused to Halo in mammalian cells

Depositor

Erika Holzbaur, Andrea Stavoe

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-7D

Plasmid

#90007

Purpose

expresses 7D mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484D, 488S to 488D, 492S to 492D,…

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-EGFP

Plasmid

#52343

Purpose

AAVS1 donor vector for inducible gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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mOrange-C1

Plasmid

#54680

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mOrange

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-7A

Plasmid

#90006

Purpose

expresses 7A mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484A, 488S to 488A, 492S to 492A,…

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pZ M2rtTA_CAGG TetON-Sox10 with GFP

Plasmid

#115241

Purpose

RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Inserts

SOX10 (SOX10 Human)
eGFP

Tags

GFP (separate polypeptide)

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Dnase1L3 pIRES-DsRed

Plasmid

#98845

Purpose

Expresses Human Dnase1L3 in mammalian cells along with DsRed

Depositor

Peter Keyel

Article

Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

Insert

Dnase1L3 (DNASE1L3 Human)

Tags

IRES DsRed

Expression

Mammalian

Mutation

long isoform, wild type

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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miniSOG-Mito-7

Plasmid

#57773

Purpose

Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Mito (COX8A Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAG303-Gal-PR50

Plasmid

#84905

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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