163,486 results

1. pAF0033 Ef1a-Pa01-2a-EGFP

   Plasmid

   #193460

   Purpose

   Expression of Pa01 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Flag hHMGB1

   Plasmid

   #31609

   Depositor

   Yasuhiko Kawakami

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4xM67 pTATA TK-Luc

   Plasmid

   #8688

   Depositor

   Jim Darnell

   Article

   Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

   Insert

   4 repeated M67 sites
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-R-iLACCO1.2

   Plasmid

   #208027

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1.2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-IGF1R-V5/HIS

   Plasmid

   #201979

   Purpose

   expression of human IGF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   IGF1R receptor tyrosine kinase (IGF1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2pA2-9xGRE

   Plasmid

   #74736

   Purpose

   GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

   Depositor

   Luisa Dalla Valle

   Article

   Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

   Insert

   EGFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   nine GC-responsive elements (9xGRE) from the rat …

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEmerald-Sec23A

   Plasmid

   #166893

   Purpose

   mammalian expression of Sec23A tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGAL4-DBD-MCS

   Plasmid

   #145245

   Purpose

   Backbone vector expressing GAL4 DNA-binding domain followed by MCS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PLKO.1-Scrambled

    Plasmid

    #136035

    Purpose

    Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    None (Scrambled)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-HA-eLACCO2.1-NGR

    Plasmid

    #208023

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Use

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

    Plasmid

    #136904

    Purpose

    Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    mScarletGo-p2a-Blas
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HSA3_His

    Plasmid

    #202765

    Purpose

    human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA3
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-E-Syt2

    Plasmid

    #66831

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge