We narrowed to 2,682 results for: KL

1. pcDNA3.1-IDH2-WT

   Plasmid

   #87926

   Purpose

   Expresses IDH2 (wild-type) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHDM-Tat1b

   Plasmid

   #164442

   Purpose

   Encodes HIV-1 Tat accessory protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   HIV-1 Tat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. B-GRAM-W

   Plasmid

   #211707

   Purpose

   Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PM-RA

   Plasmid

   #211705

   Purpose

   Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   PM-targetted RA (Lck Synthetic)
   

   Use

   Tags

   RA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-Dual-Guide-Donor-ShuttleOnly

   Plasmid

   #171098

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   XbaI site added upstream of U6 promotor. GtoC mu…

   Promoter

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. hPGK_V5-APEX2-GFP

   Plasmid

   #105285

   Purpose

   drives expression of V5-NES-APEX2-GFP from human PGK promotor

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   V5-APEX2-NES
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK promoter

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-EGFPL10a

   Plasmid

   #98747

   Purpose

   Cre-dependently expresses the fusion protein EGFPL10a

   Depositor

   Eric Schmidt, Alexander Nectow, Nathaniel Heintz

   Article

   Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

   Has Service

   AAV Retrograde and AAV5

   Insert

   EGFPL10a
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES2_EGFP_KCNQ1

   Plasmid

   #173161

   Purpose

   For mammalian expression of KCNQ1 and creating variants

   Depositor

   Al George

   Article

   Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

   Insert

   KCNQ1 (KCNQ1 Human)
   

   Use

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-Tuba

   Plasmid

   #22214

   Depositor

   Pietro De Camilli

   Article

   Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

   Insert

   Tuba (DNMBP Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

10. Zebrafish aBb crystallin 2kb promoter/AcGFP

    Plasmid

    #98099

    Purpose

    Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1-PM-RA-BlastR

    Plasmid

    #211708

    Purpose

    Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    PM-targetted RA (Lck Synthetic)
    

    Use

    Lentiviral

    Tags

    RA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HyperADAR control

    Plasmid

    #166969

    Purpose

    Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    linker-hyperADAR
    

    Use

    Retroviral

    Tags

    linker-HyperADAR

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-GW-HA-RINT1

    Plasmid

    #39841

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    RINT1 (RINT1 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX317-VRK2

    Plasmid

    #199641

    Purpose

    Expresses VRK2

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK2 (VRK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSI2-DCD

    Plasmid

    #166968

    Purpose

    Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-DCD
    

    Use

    Retroviral

    Tags

    MSI2-DCD

    Expression

    Mammalian

    Mutation

    N495S

    Promoter

    MSCV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLKO-mcherry-luc-puro-TRPM1 cDNA

    Plasmid

    #29782

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    TRPM1 (TRPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH-SFB-DYRK1A

    Plasmid

    #101770

    Purpose

    Mammalian expression of DYRK1A fusion protein

    Depositor

    Michael Huen

    Article

    Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-Ubiquitin-K29R

    Plasmid

    #17602

    Purpose

    Mammalian expression of HA tagged ubiquitin K29R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29R; V5 deleted

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSI2-ADA

    Plasmid

    #166967

    Purpose

    Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

    Depositor

    Michael Kharas

    Article

    Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

    Insert

    MSI2-ADA
    

    Use

    Retroviral

    Tags

    MSI2-ADA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK5 FLAG wildtype, catalytic active lipin 1

    Plasmid

    #32005

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLJM1-B-GRAM-W-NeoR

    Plasmid

    #211710

    Purpose

    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Tryptophan

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-HA-MST2

    Plasmid

    #33098

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    MST2 (STK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1-IDH2-R172K

    Plasmid

    #87927

    Purpose

    Expresses IDH2 (R172K mutant) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    R172K

    Promoter

    CMV

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMZ7

    Plasmid

    #223180

    Purpose

    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

    Depositor

    Paul Blainey

    Article

    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

    Type

    Empty backbone

    Use

    Chromosomal integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5FRT_KCNQ3

    Plasmid

    #173163

    Purpose

    To generate Flp-In CHO-KCNQ3 stable cells

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLJM1-B-GRAM-H-NeoR

    Plasmid

    #211709

    Purpose

    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBLO 62.4

    Plasmid

    #123123

    Purpose

    Human expression vector for deltaproteobacteria CasX and sgRNA

    Depositor

    Benjamin Oakes, Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Inserts

    CasX protein
    CasX sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

    Plasmid

    #110302

    Purpose

    Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge