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Plasmid#123193Purposebinary plant vector for transient TEV HcPro and fLUC (with intron) expressionDepositorInsert2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFAB815
Plasmid#47854PurposeBIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyDepositorInsertrnpB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO9Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
Plasmid#127560PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertCas9-T2A-GFP
UseCRISPRTagsExpressionInsectMutationEGFP-D102G see depositor comments belowPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH18
Plasmid#154397PurposeDerived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9DepositorInsertFused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNAAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-eGFP.PB/PTK
Plasmid#170540PurposeExpresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restoredDepositorInsertDelta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.0
Plasmid#149429PurposeminiTdg geneDepositorInsertTdg (Tdg Mouse)
UseCre/LoxTagsExpressionMammalianMutationSNPs of the Tdg gene from the OLA/129 strainPromoterTdg promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)
Plasmid#55199PurposeCFP reporter along with FF4 miRNA binding sites and 28 nt sequencesDepositorInsertECFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)
Plasmid#164046PurposeCRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA geneDepositorInsertmTagBFG-TriTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
221-RPB1-AID-eGFP-2A-blasticidin
Plasmid#195107PurposeTargeting vector to introduce AID-eGFP tags in C-terminal of the human RPB1 gene. Blasticidin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertAID-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-hNgAgo
Plasmid#104384Purposehuman codon optimized NgAgo; pCDNA3 vector backbone, mammalian expressionDepositorInserthNgAgo
UseTags3XFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
XW17
Plasmid#65835PurposePunc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsmCherryExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB115 - pL2_pSB90_fLUC-I_tGFP-I
Plasmid#123190Purposebinary plant vector for transient tGFP (with intron) and fLUC (with intron) expressionDepositorInsert2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFAB822
Plasmid#47856PurposeBIOFAB reporter plasmid for measuring BBa_B1006 termination efficiencyDepositorInsertBBa_B1006 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO11Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB803
Plasmid#47847PurposeBIOFAB reporter plasmid for measuring T7 early termination efficiencyDepositorInsertT7 early terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO2Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-OsMIR390-B/c
Plasmid#61468PurposeGateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.DepositorInsertOsMIR390-B/c
UseGateway entry vectorTagsExpressionMutationO. sativa MIR390 precursor sequence including a c…PromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS
Plasmid#123188Purposebinary plant vector for transient fLUC (with intron) expressionDepositorInsert2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dGZmxh
Plasmid#44552DepositorInsertsPTETREG promoter
yEGFP::ZeoR
UseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationPromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-A
Plasmid#64513PurposeAS-30D hepatoma hexokinase II 4.3 kbp promoter-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase IIAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-C
Plasmid#64515PurposeAS-30D hepatoma hexokinase II 2.1 kbp promoter-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase IIAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-E
Plasmid#64517PurposeAS-30D hepatoma hexokinase II 0.1 kbp promoter-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase IIAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Btg1
Plasmid#45865DepositorInsertBtg1 in situ probe (Btg1 Mouse)
UseIn situTagsExpressionMutationcontains bp# 1004-1638 of BC006834.1PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3906
Plasmid#47809PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.DepositorInsertpromoter 14 and BCD2
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235268PurposeLentiviral expression of ComMAND base gene regulating mRuby2DepositorInsertmRuby2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G (3rd-generation Tet system)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
SRGAP2C_pEF-DEST51
Plasmid#238497PurposeIVT mRNA of human geneDepositorInsertSRGAP2C (SRGAP2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
ARHGAP11B_pEF-DEST51
Plasmid#238498PurposeIVT mRNA of human geneDepositorInsertARHGAP11B (ARHGAP11B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
GPR89B_full_pGCS1
Plasmid#238499PurposeIVT mRNA of human geneDepositorInsertGPR89B (GPR89B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-Fgf5-t2A-eGFP
Plasmid#227504PurposeInserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassetteDepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pGEMT-mMyh7CDS
Plasmid#192849PurposePlasmid to produce ISH probe for mMyh7 gene with CDS sequenceDepositorInsertmyosin heavy chain 7 (Myh7 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh7UTR
Plasmid#192850PurposePlasmid to produce ISH probe for mMyh7 gene with UTR sequenceDepositorInsertmyosin heavy chain 7 (Myh7 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF702
Plasmid#209650PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicusDepositorInsertsDownstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Gja1prom
Plasmid#188114PurposeExpresses luciferase under control of Gja1 promoter in transfected cellsDepositorInsertGja1 promoter (Gja1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterGja1 endogenous promoter up to gene start codonAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-LS
Plasmid#205438PurposeExpression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genesDepositorInsertsGaussia luciferase gene
spectinomycin resistance gene
UseCre/Lox and Luciferase; Chlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_P1
Plasmid#124455PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_P1_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_P2
Plasmid#124456PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_P2_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_P3
Plasmid#124457PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_P3_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_P4
Plasmid#124458PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_P4_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_P5
Plasmid#124459PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_P5_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_E2-1
Plasmid#124474PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_E2-1_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_E2-2
Plasmid#124475PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_E2-2_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_E2-3
Plasmid#124476PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_E2-3_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_E2-4
Plasmid#124477PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_E2-4_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA_BLK_E3-3
Plasmid#124484PurposeFor CRISPR-mediated epigenome editing of human BLK gene locusDepositorInsertBLK_E3-3_gRNA (BLK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLD-CAG-DYSF-CPL
Plasmid#131458PurposeEncodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B along with luciferase enzyme for in vivo live imagingDepositorInsertDYSF (DYSF Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)
Plasmid#164047PurposeCRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA geneDepositorInsertmCherry-TriTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB153 - pL2_pSB90_2x35S::ZCT1::tMAS
Plasmid#123195Purposebinary plant vector for transient expression of ZCT1 from Catharanthus roseusDepositorInsert2x35S::ZCT1::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS
Plasmid#123196Purposebinary plant vector for transient expression of ORCA3 from Catharanthus roseusDepositorInsert2x35S::ORCA3::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS
Plasmid#123199Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInserttMAS::rLUC-I::pMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Zeo-NKX2-8
Plasmid#75090Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorInsertNKX2-8 (NKX2-8 Human)
UseRetroviralTagsnoneExpressionMammalianMutationwild-type, full-length, without native 3' UTRPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only