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Plasmid#126149PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23116Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbE9k-RFP
Plasmid#126148PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23113Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB20k-GFP
Plasmid#126136PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterS23200Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB15k-GFP
Plasmid#126134PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB14k-GFP
Plasmid#126123PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB12k-GFP
Plasmid#126121PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23151Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB11k-GFP
Plasmid#126120PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23150Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB10k-GFP
Plasmid#126119PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23116Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB9k-GFP
Plasmid#126118PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23113Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA18k-RFP
Plasmid#126107PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23117Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA20k-RFP
Plasmid#126106PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterS23200Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA19k-RFP
Plasmid#126105PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA15k-RFP
Plasmid#126104PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA13k-RFP
Plasmid#126092PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23102Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA12k-RFP
Plasmid#126091PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23151Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA11k-RFP
Plasmid#126090PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23150Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA10k-RFP
Plasmid#126089PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23116Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA9k-RFP
Plasmid#126088PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23113Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-194-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85929PurposeSensor PlasmidDepositorInsertmiR-194-3p Sensor
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-526a.sensor.PGK.dNGFR.WPRE
Plasmid#85900PurposeSensor PlasmidDepositorInsertmiR-526a Sensor
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-486-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85883PurposeSensor PlasmidDepositorInsertmiR-486-3p Sensor
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-301b.sensor.PGK.dNGFR.WPRE
Plasmid#85850PurposeSensor PlasmidDepositorInsertmir-301b Sensor
UseLentiviralTags-ExpressionMutationPromoterPGKAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-181a-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85871PurposeSensor PlasmidDepositorInsertmiR-181a-3p Sensor
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-425-5p.sensor.PGK.dNGFR.WPRE
Plasmid#85858PurposeSensor PlasmidDepositorInsertmiR-425-5p Sensor
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-512-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85879PurposeSensor PlasmidDepositorInsertmiR-512-3p Sensor
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
ARK2a
Plasmid#81017PurposeE.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco
PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA:Tyr (albino)
Plasmid#60966PurposeExpression vector of rat Tyr (albino) guide RNADepositorInsertTyrosinase gRNA
UseCRISPRTagsExpressionMutationPromoterT7Available sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA:Tyr (wild)
Plasmid#60967PurposeExpression vector of rat Tyr (wild) guide RNADepositorInsertTyrosinase gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Δ741-752Δconst-GFP
Plasmid#33120DepositorInsertPcdh-γA3 (Pcdhga3 Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion of aa 741-752 along with the entire cons…PromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 myc CIITA (P#808)
Plasmid#14650DepositorInsertCIITA (CIITA Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1_AGIA-AirID
Plasmid#182210PurposeExpress AirID in mammalian cells.DepositorInsertAGIA-AirID
UseTagsAGIA-tagExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PE-P3-rice
Plasmid#185612PurposeFor plant prime editing in rice plantsDepositorInsert3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luke-2
Plasmid#228900PurposeLuke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertCarbohydrate binding domain cbm49 protein
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-LRP6c
Plasmid#42960PurposeExpresses optogenetically controlled LRP6 (C-terminal domain)DepositorInsertCry2PHR-mCherry-LRP6c
UseTagsExpressionMammalianMutationLPR6 cytoplasmic domain onlyPromoterCMVAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106TagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK462 (Hygro-P2A-mAB)
Plasmid#214388PurposemAID-BromoTag for N-terminal taggingDepositorInsertHygro-P2A-mAID-BromoTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11-DI-GFP1–9
Plasmid#108260PurposeBinary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT57
Plasmid#223429PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK445 (BSD-P2A-BromoTag)
Plasmid#214375PurposeBromoTag for N-terminal taggingDepositorInsertBSD-P2A-BromoTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pADV-Sec61b-TurboID
Plasmid#179143PurposeRecombinant adenoviral plasmid for Sec61b-TurboID expressionDepositorInsertSec61b-V5-TurboID
UseAdenoviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only