We narrowed to 1,093 results for: Amot;
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Plasmid#212022PurposeFor labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].DepositorInsertGianCreg (GOLGB1 Human)
UseTagstd5StayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
ALCAM-p2A-E2Crimson
Plasmid#83939PurposeLentiviral vector expressing ALCAM-p2A-E2CrimsonDepositorInsertALCAM (ALCAM Human)
UseLentiviralTagsp2A-E2CrimsonExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIG-601_NY-ESO-1_TCR_GFP
Plasmid#207482PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertGFP, NY-ESO-1_TCR
UseCRISPRTagsExpressionMutationPotential silent point mutation in GFP sequencePromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]
Plasmid#190236PurposeAAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinaseDepositorInsertEGFP
UseAAVTagsF2A, LDLR C-terminal sequence, mRFP1, myristoylat…ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGmCherry-gRNA
Plasmid#87110PurposegRNA cloning vector with CAGmCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Neo)
Plasmid#140645PurposeCTCF tagging with mAID-CloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHL-H1-ccdB-mEF1a-RiH
Plasmid#60601PurposeCloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.DepositorInsertsRed Fluorescent Protein
Hygromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9-iP-A
Plasmid#60599PurposeExpresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertCRISPR Cas9
UseCRISPRTagsExpressionMammalianMutationCodon usage optimized for human usagePromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)(W33A)
Plasmid#221761PurposeStable expression of GFP-CERT(PHD)(W33A) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116, mutated 33W to 33APromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-HA-mBec1 F121A
Plasmid#99506PurposeLentiviral expression of mutant Becn1 leading to hyperactive autophagyDepositorInsertBeclin 1 (Becn1 Mouse)
UseLentiviralTagsHAExpressionMutationF121APromoterCMVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-gSM
Plasmid#215870PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInsert3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-gCM
Plasmid#215868PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInserttarget site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mCherry-p115(S240A)
Plasmid#181997PurposeMammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.DepositorInsertmCherry-p115(S240A) (ARHGEF1 Human)
UseTagsmCherryExpressionMammalianMutationSerine 240 changed to alanine in p115.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-747_CD19-28z_CAR_TFAP4
Plasmid#207489PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, CD19-28z_CAR (TFAP4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI4-3×FLAG
Plasmid#215512PurposeExpresses FLAG tagged human WIPI4.DepositorInsertWD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)
UseRetroviralTags3×FLAGExpressionMammalianMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-AMPKalpha2 M93G
Plasmid#31653DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
UseTagsM2ExpressionMammalianMutationchanged Methionine 93 to GlycinePromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pECE-HA-AMPKalpha2 M93G
Plasmid#31655DepositorInsertprotein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationchanged Methionine 93 to GlycinePromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP-plk1-AKAP
Plasmid#132967PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorUseTagsExpressionMammalianMutationC terminus of full-length Plk1 fused to the peric…PromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHC1 CRISPR
Plasmid#140545PurposeDHC1 tagging CRISPRDepositorInsertDHC1 (DYNC1H1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only