We narrowed to 2,847 results for: PHI-1

1. pUC19-whiteCopyCatcher

   Plasmid

   #174062

   Purpose

   CopyCatcher insertion donor for the white locus

   Depositor

   Ethan Bier

   Article

   Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

   Insert

   white (w Fly, Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV0757

   Plasmid

   #133509

   Purpose

   Expression of 3GFP tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-3XP3.RFP-tan

   Plasmid

   #83474

   Purpose

   RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   3XP3.RFP-tan
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   3XP3 eye-expression Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXint129

   Plasmid

   #178208

   Purpose

   Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

   Depositor

   Gregory Phillips

   Article

   Platt et al Plasmid. 2000 Jan . 43(1):12-23.

   Insert

   xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacI

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-WingGFP-tan

   Plasmid

   #83473

   Purpose

   EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   WingGFP-tan
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Hsp70 and enhancer from D. melanogaster yellow ge…

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV0756

   Plasmid

   #133508

   Purpose

   Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_NSnoFbox-vhhGFP4

   Plasmid

   #35580

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSnoFbox-vhhGFP4 (slmb Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F box domain of slmb deleted.

   Promoter

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-12x

   Plasmid

   #61699

   Purpose

   Expresses PTDH mutant Opt12x

   Depositor

   Huimin Zhao

   Article

   Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

   Insert

   Opt12x
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRVV210

   Plasmid

   #87628

   Purpose

   Drives Bxb1 expression in the D. melanogaster germ line.

   Depositor

   Richard Mann

   Article

   Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

   Insert

   Bxb1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   vasa

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPgCdH3

    Plasmid

    #8695

    Depositor

    Kami Ahmad

    Article

    Ahmad et al J Cell Biol 2001 Apr 2;153(1):101-10.

    Insert

    cid promoter, H3 (His3:CG31613 Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57mini ZM249M P90A _env eGFP

    Plasmid

    #132939

    Purpose

    pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    HIV-1ZM249M provirus (P90A) with env deletion, GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    P90A

    Promoter

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPU-Empty-miR30-TRIM5

    Plasmid

    #132936

    Purpose

    All-in-one control rescue-TRIM5 shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    control shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. KHBD00246

    Plasmid

    #34381

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG2711 (dwg Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pAc-GFP-dSUMO

    Plasmid

    #85696

    Purpose

    expression of GFP-SUMO1 in Drosophila cells

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pTTCC

    Plasmid

    #164264

    Purpose

    Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

    Depositor

    Gudmundur Hreggvidsson

    Article

    Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

    Inserts

    Cas9
    gRNA scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcel and trc

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF-5xT7-TtCsm

    Plasmid

    #128572

    Purpose

    For expression of T. thermophilus Csm in E. coli (Csm1-5)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Csm1
    Csm2, Csm3, Csm4, Csm5
    

    Use

    Tags

    HRV 3C protease cleavage site and 10xHis tag on C…

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli and Cod…

    Promoter

    T7 promoter, lac operator

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1C-Fluc-CG9441_E

    Plasmid

    #146246

    Purpose

    Insect Expression of CG9441-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jan . 15(1):21-32.

    Insert

    CG9441-3UTR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPP076

    Plasmid

    #158794

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPP378

    Plasmid

    #158797

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-1 (D371A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRVV651

    Plasmid

    #87631

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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