Skip to main content
Addgene
Showing: 961 - 980 of 13534 results
  1. pCRISPR-cBEST

    Plasmid
    #125689
    Purpose
    C to T base editor for actinomycetes
    Depositor
    Insert
    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    ermE*/tipA
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pCMV-Popt

    Plasmid
    #231705
    Purpose
    Mammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.
    Depositor
    Insert
    VSV P gene (codon-optimized )
    Use
    Vsv expression
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pCMV-Lopt

    Plasmid
    #231706
    Purpose
    Mammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.
    Depositor
    Insert
    VSV L gene (codon-optimized )
    Use
    Vsv expression
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-CAG-LiLac

    Plasmid
    #184570
    Purpose
    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV
    Depositor
    Insert
    LiLac fluorescent sensor of lactate
    Use
    AAV
    Tags
    His7
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pIMVAu1

    Plasmid
    #98301
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.
    Depositor
    Insert
    HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pAD-KPS12-Pman

    Plasmid
    #133832
    Purpose
    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Insert
    mKate(KPS12)
    Use
    E.coli/bacillus spp. shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pman
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Insert
    MevT and MBIS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 961 - 980 of 13534 results