We narrowed to 4,333 results for: U6 gRNA

1. pgRNA_hMECP2_promoter_No9

   Plasmid

   #194903

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNA_hMECP2_promoter_No4

   Plasmid

   #194899

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA1_NANOG

   Plasmid

   #64153

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA4_NANOG

   Plasmid

   #64156

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA4_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA3_NANOG

   Plasmid

   #64155

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA2_NANOG

   Plasmid

   #64154

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA_SOX2_HDR

   Plasmid

   #163752

   Purpose

   gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   gRNA_SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pgRNA_hMECP2_promoter_No8

   Plasmid

   #194902

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.8
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pgRNA_hMECP2_promoter_No6

   Plasmid

   #194900

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.6
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pgRNA_hMECP2_promoter_No7

    Plasmid

    #194901

    Purpose

    Lentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Insert

    sgRNA targeting human MECP2 promoter No.7
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgRNA_CV2

    Plasmid

    #167165

    Purpose

    pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

    Depositor

    Alexander Loewer

    Article

    Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AM77_pU6.Sa-gRNA.CLYBL

    Plasmid

    #199237

    Purpose

    Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. aureus gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid

    #199258

    Purpose

    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AV15_pCAG.Cas9.gRNA.S1

    Plasmid

    #199259

    Purpose

    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-sgRNA_Dicer_2

    Plasmid

    #68808

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AV13_pCAG.Cas9.gRNA.NT

    Plasmid

    #199260

    Purpose

    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC1812_pCR8-gCasRx-CUG (control gRNA)

    Plasmid

    #118645

    Purpose

    Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gCasRx-CUG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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