We narrowed to 88,746 results for: Mal

1. pJZC77

   Plasmid

   #62338

   Purpose

   sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA
   COM-KRAB
   

   Use

   Lentiviral

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

   Promoter

   CMV and U6

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. DPYS-FLAG

   Plasmid

   #61618

   Purpose

   To study the effect of DPYS activity on EMT

   Depositor

   David Sabatini

   Article

   Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

   Insert

   dihydropyrimidinase (DPYS Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRY-tTA (B702)

   Plasmid

   #92032

   Purpose

   Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Tags

   tTA2 (TetR fused to VP64)

   Expression

   Mammalian

   Mutation

   NLS on CRY2 is mutated

   Promoter

   CMV

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual-eGFP(Stop66)

   Plasmid

   #63218

   Purpose

   Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(Stop66)
   

   Use

   Lentiviral

   Tags

   T7 and his6

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. D998-EGFR-ET1

   Plasmid

   #44186

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   D998-EGFR-ET1 (EGFR Human)
   

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   EGFR amino acids 1−998

   Promoter

   CVM

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCOCK CMVwt

   Plasmid

   #46559

   Purpose

   Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

   Insert

   CMV wt promoter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   Our version has a G at base 724 (reference has C)

   Promoter

   CMV

   Available Since

   Sept. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJZC73

   Plasmid

   #62341

   Purpose

   sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA
   COM-KRAB
   

   Use

   Lentiviral

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

   Promoter

   CMV and U6

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCOCK CM-R05

   Plasmid

   #46568

   Purpose

   Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).

   Depositor

   Clifford Wang

   Article

   Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

   Insert

   CMV mutant promoter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   Mutant version of CMV promoter (refer to Table 1 …

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pDual-eGFP(W66)

   Plasmid

   #63217

   Purpose

   Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(W66)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 PA-mCit-NL

    Plasmid

    #113444

    Purpose

    ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. MO70-hJunctate - EF-mutant

    Plasmid

    #79597

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    77DAD79 to 77AAA79

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA

    Plasmid

    #108831

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1 tTA SDHA

    Plasmid

    #177976

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    SDHA (SDHA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Silent point mutations to SDHA sgRNA target

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBp-FGFR2c-W290C

    Plasmid

    #45701

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    W290C

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAG69 G6PD 3'UTR mut

    Plasmid

    #12053

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    G6PD 3'UTR mut (G6PD Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBp-FGFR2b-P253R

    Plasmid

    #45716

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    P253R

    Available Since

    June 27, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLenti PA-mCit-GW

    Plasmid

    #113456

    Purpose

    Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ProteinA-mCitrine

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_SNORD27h_115mut

    Plasmid

    #73068

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27 with AS box from …

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pBW2701_pCAG-PV1-iCre-N270-L1-nMag-NLS-BGHpA

    Plasmid

    #108823

    Purpose

    Expresses N-terminus to aa270 of Cre recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa270
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBW2702_pCAG-PV1-pMag-L1-iCre-271C-BGHpA

    Plasmid

    #108824

    Purpose

    Expresses aa271 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa271 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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