We narrowed to 88,262 results for: MAL

1. pET21b-Rac1/13C-FKBP-MBP

   Plasmid

   #214295

   Purpose

   Express split-Rac1 fragment fused with FKBP and MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Split-Rac1 fragment fused with FKBP (RAC1 Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Rac1-Q61L

   Promoter

   T7

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21b-RhoA/13C-FKBP-MBP

   Plasmid

   #214299

   Purpose

   Express split-RhoA fragment fused with FKBP and MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Split-RhoA fragment fused with FKBP (RHOA Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   RhoA-Q63L

   Promoter

   T7

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTFG011_Spike_Parental_D614G_3xFlag

   Plasmid

   #191571

   Purpose

   SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   SARS-CoV-2 Spike Protein (S Synthetic)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   D614G mutation. Last 19 amino acids truncated.

   Promoter

   CMV

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-SPNS1-sg1

   Plasmid

   #198566

   Purpose

   SPNS1-targeting sgRNA in PX458 vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1-sg1 (SPNS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-SPNS1-sg2

   Plasmid

   #198567

   Purpose

   SPNS1-targeting sgRNA in PX458 vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1-sg2 (SPNS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-G12-hFGF1

   Plasmid

   #219554

   Purpose

   hFGF1 expression in mammalian cells. pAAV vector. Reporter plasmid.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   hFGF1 (FGF1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   12xUAS with TATA-box minimal promoter

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

   Plasmid

   #214882

   Purpose

   Lentiviral vector encoding RfxCas13d targeting PROX guide array

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crPROX-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR-cBEST-v2-SF14P

   Plasmid

   #209448

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from SF14P promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR-cBEST-v2-kasOP*

   Plasmid

   #209447

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from kasOP* promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-dCas12a-AID

    Plasmid

    #193647

    Purpose

    Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hAID, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-dCas12a-BE

    Plasmid

    #193648

    Purpose

    Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-rAPOBEC1, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-dCas12a-CDA1

    Plasmid

    #193649

    Purpose

    Expression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-PmCDA1, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-dCas12a-eAID

    Plasmid

    #193650

    Purpose

    Expression of base editor dCas12a-eAID in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-eAID, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-VcnTS-E1015A-E1021A

    Plasmid

    #213417

    Purpose

    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid

    #213414

    Purpose

    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-SUMO-A'-l2-A'-IRES-EmGFP

    Plasmid

    #209125

    Purpose

    Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'-l2-A'-IRES-EmGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. phROSA26-Tet-HA-JLP_V55Q

    Plasmid

    #208771

    Purpose

    Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JLP (SPAG9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

    Plasmid

    #174895

    Purpose

    amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FKBP12-CB2mutx2-mTagBFP2-HAx2

    Plasmid

    #207147

    Purpose

    Tandem mutant calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Use

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Mutation

    E40Q, E87Q, E131Q, E175Q, E219Q

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

    Plasmid

    #200639

    Purpose

    Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge