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Plasmid#226311PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-7
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB55
Plasmid#226310PurposeE. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.DepositorInsertCsoS2 with V(T/S)G to AAA CsoS2 MR1-5
UseTagsExpressionBacterialMutationVTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…PromoterpTRCAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG068
Plasmid#214259PurposepJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaIDepositorInsertCore genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
UseTagsExpressionBacterialMutation18 nucleotide 5' of the start codon (Shine-D…PromoteraraBAD promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG059
Plasmid#214262PurposePaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)DepositorInserttLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
UseTags6x His-tag and 6xHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)
Plasmid#224490PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-iRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)
Plasmid#224491PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-tdiRFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)
Plasmid#224524PurposeA Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassetteDepositorInsertrox-3xNLS-mCherry-V5-2xSTOP-rox
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E lexOP-c-fos min pro (JDW 1254)
Plasmid#224477PurposeGateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoterDepositorInsertLexOp
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-dr_Unc (JDW 1365)
Plasmid#224482PurposeGateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intronDepositorInsertUnc-503 promoter
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-V5-n2StayGoldC4 (JDW 1354)
Plasmid#224486PurposeGateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)DepositorInsertH2B-V5-n2StayGoldC4
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-mCherry-3xHA (JDW 1321)
Plasmid#224493PurposeGateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)DepositorInsertLifeact-mCherry-3xHA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME Lamin B Vhh mNeonGreen-pA (JDW 1312)
Plasmid#224501PurposeGateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)DepositorInsertLamin B Vhh mNeonGreen-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME TAEL2.0-5xC120-minpro (JDW 1109)
Plasmid#224507PurposeGateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)DepositorInsertTAEL2.0-5xC120
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-TAEL (JDW 1324)
Plasmid#224508PurposeGateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)DepositorInsertTAEL
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS2 (JDW 1209)
Plasmid#224511PurposeA Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCSDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP-Cre (JDW 1329)
Plasmid#224515PurposeA Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusionDepositorInsertnls-EGFP-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME iCre-I (JDW 1203)
Plasmid#224520PurposeA Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteriaDepositorInsertiCre-I
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)
Plasmid#224532PurposeA Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyADepositorInsertIRES-H2A-mCherry-SV40pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E EFS-rtTA/rtTA3 (JDW 1214)
Plasmid#224533PurposeA Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivatorDepositorInsertrtTA
UseGateway cloningTagsExpressionMutationPromoterEFSAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)
Plasmid#224534PurposeA Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tagDepositorInsertActin-Vhh-mNeonGreen-HA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only